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Effect of Sperm Dna Damage and Sperm Protamine Deficiency on Fertilization and Embryo Development Post-Icsi Publisher Pubmed



Nasresfahani MH1, 2 ; Salehi M3 ; Razavi S3 ; Anjomshoa M3 ; Rozbahani S4 ; Moulavi F1 ; Mardani M3
Authors
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Authors Affiliations
  1. 1. Department of Clinical and Experimental Embryology and Andrology, Royan Institute, Tehran, Iran
  2. 2. Isfahan Fertility and Infertility Centre, Isfahan, Iran
  3. 3. Department of Anatomy, Isfahan Medical University, Isfahan, Iran
  4. 4. Islamic Azad University of Falavarjan, Falavarjan, Iran

Source: Reproductive BioMedicine Online Published:2005


Abstract

The aim of this study was to evaluate the effect of sperm DNA damage and protamine deficiency on fertilization and embryo development post-intracytoplasmic sperm injection (ICSI), and also to assess the effect of protamine deficiency on DNA damage. Semen samples were collected from 28 patients participating in the ICSI programme. Following sperm preparation and ICSI, the remaining processed semen samples were used to assess protamine deficiency and DNA damage employing chromomycin A3 (CMA3) staining and comet assay, respectively. Comet parameters, CMA3 percentage positivity, fertilization rate, embryo cleavage score and embryo quality score were assessed. Except for CMA3, none of the comet parameters showed significant correlation with fertilization rate. However, among comet parameters, head area and head intensity showed positive correlation with the embryo cleavage score, while comet mean intensity and head mean intensity showed a significant negative correlation with CMA3 positivity. Results of this study demonstrate that DNA fragmentation is more frequent in protamine-deficient spermatozoa. Unlike protamine deficiency, sperm DNA fragmentation does not preclude fertilization. Nonetheless, embryos derived from spermatozoa with high DNA damage have a lower potential to reach blastocyst stage.
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