Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effects of Lactobacillus Plantarum A7 With Probiotic Potential on Colon Cancer and Normal Cells Proliferation in Comparison With a Commercial Strain



Sadeghialiabadi H1 ; Mohammadi F1 ; Fazeli H2 ; Mirlohi M3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Microbiology, Medical School, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Food Security Research Center, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Iranian Journal of Basic Medical Sciences Published:2014

Abstract

Objective(s): Several beneficial effects have been attributed to the probiotic lactic acid bacteria. It was determined that lactobacilli can exert antiproliferative effects on the various cancer cell lines including colon cancer. Effects of lactic acid bacteria on colon cancer may vary from strain to strain and there is a need to find the new probiotic strains with tumor suppressing properties through in vitro studies.; Materials and Methods: Anti-proliferative activities of heat-killed cells and cell-free supernatants of a native strain of Lactobacillus plantarum A7 and a commercial strain of lactobacillus rhamnosus GG were assessed on human colon cancer cell lines (Caco-2 and HT-29) and normal cells (L-929), using MTT assay. Cells were seeded at 2×104 cells/mlin 96 well plates and incubated for 24 hr. Then heat-killed cells (OD620: 0.025, 0.0.05, 0.1) and cell-free supernatants of bacteria were added at concentration of 2.5, 5 and 10 mg/ml. After 48 hr incubation MTT (5 mg/ml) was added and the absorbance was measured at 540 nm using ELISA plate reader.; Results: Results showed that heat-killed cells and cell-free supernatants of both probiotic strains reduced the growth rate of cancer and normal cells. These results suggested that anti-proliferative effect may not be an exclusive characteris ticwhich is dedicated to officially approved probiotics.; Conclusion: L. plantarum A7 could be considered as colon cancer biological product, most likely due to its advantages in significant organic acid production. © 2014, Mashhad University of Medical Sciences. All rights reserved.
Related Docs
View other Related Docs
1. Prophylactic Effects of Secretion Metabolites of Dairy Lactobacilli Through Downregulation of Erbb-2 and Erbb-3 Genes on Colon Cancer Cells, European Journal of Cancer Prevention (2020)
2. Veillonella Parvula As an Anaerobic Lactate-Fermenting Bacterium for Inhibition of Tumor Growth and Metastasis Through Tumor-Specific Colonization and Decrease of Tumor’S Lactate Level, Scientific Reports (2024)
3. Nisin Induces Apoptosis in Cervical Cancer Cells Via Reactive Oxygen Species Generation and Mitochondrial Membrane Potential Changes, Biochemistry and Cell Biology (2022)
4. Probiotic Potential, Antiproliferative, and Proapoptotic Activities of Lactobacillus Fermentum Isolated From Breast Milk, Advanced Biomedical Research (2023)
5. Stability Evaluation of Freeze-Dried Lactobacillus Paracasei Subsp. Tolerance and Lactobacillus Delbrueckii Subsp. Bulgaricus in Oral Capsules, Research in Pharmaceutical Sciences (2012)
6. In Vitro Anti-Bacterial and Anti-Adherence Effects of Lactobacillus Delbrueckii Subsp Bulgaricus on Escherichia Coli, Research in Pharmaceutical Sciences (2013)
7. Technology and Potential Applications of Probiotic Encapsulation in Fermented Milk Products, Journal of Food Science and Technology (2015)
8. Role of Gut Microbiota in the Pathogenesis of Colorectal Cancer; a Review Article, Gastroenterology and Hepatology from Bed to Bench (2018)
Experts (# of related papers)
View all Related Experts
Vajihe Akbari (3)
Sharareh Moghim (3)
Other Related Docs
9. Kefir: A Powerful Probiotics With Anticancer Properties, Medical Oncology (2017)
10. Effects of Probiotics With or Without Honey on Radiation-Induced Diarrhea, International Journal of Radiation Research (2016)
11. The Effect of Intestinal Microbiota Metabolites on Ht29 Cell Line Using Mtt Method in Patients With Colorectal Cancer, Gastroenterology and Hepatology from Bed to Bench (2019)
12. In Vitro Selective Growth Inhibition of Breast Adenocarcinoma Cell Lines by Pseudomonas Sp. Uw4 Metabolites, Tehran University Medical Journal (2016)
13. Effect of a Dietary Supplement Composed of Hydrolyzed Milk Proteins and Vanillin on the Reduction of Infection and Oxidative Stress Induced by Chemotherapy, Journal of Food Biochemistry (2020)
14. Alterations in the Gut Microbiota and Their Metabolites in Human Intestinal Epithelial Cells of Patients With Colorectal Cancer, Molecular Biology Reports (2024)
15. Effect of Supplements: Probiotics and Probiotic Plus Honey on Blood Cell Counts and Serum Iga in Patients Receiving Pelvic Radiotherapy, Journal of Research in Medical Sciences (2015)
16. Lactobacillus Plantarum A7, a Potential Probiotic Strain From Infant Fecal Flora, Journal of Biology and Today's World (2017)
17. Effects of Biosurfactants Derived From Lactobacillus Casei and Lactobacillus Reuteri on Gene Expression Profile of Gtfb/C and Ftf Genes in Lactobacillus Mutans, Journal of Isfahan Medical School (2014)
18. Lactobacillus Rhamnosus Biosurfactant Inhibits Biofilm Formation and Gene Expression of Caries-Inducing Streptococcus Mutans, Dental Research Journal (2019)
19. In Vitro Cytotoxic Activity of a Lactococcus Lactis Antimicrobial Peptide Against Breast Cancer Cells, Iranian Journal of Biotechnology (2018)
20. Kefir and Cancer: A Systematic Review of Literatures, Archives of Iranian Medicine (2015)
21. Microbial and Physiochemical Changes in Yoghurts Containing Different Lactobacillus Delbrueckii Subsp. Bulgaricus Strains in Association With Lactobacillus Plantarum As an Adjunct Culture, International Journal of Dairy Technology (2014)
22. Evaluation of the Simultaneous Effects of Lactobacillus Delbrueckii and Lactobacillus Lactis on Biofilms of Isolates From Chronic Ulcer Infections With Multiple-Drug Resistance, Jundishapur Journal of Microbiology (2022)
23. Inhibitory Effects of Lactococcus Lactis and Its Supernatant on Listeria Monocytogenes, Clinical Laboratory (2021)
24. Enumeration of Lactobacilli in the Fecal Flora of Infant Using Two Different Modified De-Man Rogosa Sharpe Media Under Aerobic and Anaerobic Incubation, Pakistan Journal of Biological Sciences (2008)