Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluating Inhibitory Effects of Paclitaxel and Vitamin D3 Loaded Poly Lactic Glycolic Acid Co-Delivery Nanoparticles on the Breast Cancer Cell Line Publisher



Khodaverdi S1 ; Jafari A2, 3 ; Movahedzadeh F4, 5 ; Madani F6 ; Avarvand AY7 ; Falahatkar S2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Obstetrics and Gynecology, Fellowship of Laparoscopy, Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  3. 3. Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  4. 4. Institute for Tuberculosis Research, College of Pharmacy, University of Illinois at Chicago, Chicago, IL, United States
  5. 5. Department of Pharmaceutical Sciences, College of Pharmacy University of Illinois at Chicago, Chicago, IL, United States
  6. 6. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Source: Advanced Pharmaceutical Bulletin Published:2020


Abstract

Purpose: Paclitaxel (PTX) has transpired as a significant agent in the treatment of breast cancer. Meanwhile, polylactic glycolic acid (PLGA) nanoparticles (NPs) are able to increase the anticancer effect of the PTX in the blood. Methods: Nano-precipitation was used to prepare the PLGA-PTX-VitD3 co-delivery NPs. Drug loading, encapsulation efficiency, in vitro release profile, cell viability, migration, apoptosis, and bcl2 expression of NPs were evaluated. Results: The average size of co-delivery NPs was 231 ± 46 nm. Observed was a controlled release of the PTX and vitamin D3 from co-delivery NPs between 0.5 and 240 hours. MTT showed the ability of 8 μg.mL-1 of co-delivery NPs to kill 50 % of the MCF-7; likewise, the co-delivery NPs prevented MCF-7 migration. The co-delivery NPs led 46.35 % MCF-7 to enter primary apoptosis. 60.8% of MCF-7 in the control group were able to enter the G (1) phase of the cell cycle. The co-delivery NPs increased expression of bax. In addition to its higher toxicity against MCF-7 than that of PTX, co-delivery NPs were able to release drugs continuously for a long period, which indeed increased the efficiency of the drugs. Conclusion: The effect of co-delivery NPs on MCF-7 cell viability was different from that in other drugs. In fact, the co-deliver NPs were able to release drugs continuously for a long time, this could induce primary apoptosis in the MCF-7 and decrease the metastasis and toxicity of drugs. © 2020 The Author (s).
Related Docs
1. Polymeric Nanoparticles As a Promising Platform for Treating Triple-Negative Breast Cancer: Current Status and Future Perspectives, International Journal of Pharmaceutics (2024)
Experts (# of related papers)
Nima Rezaei (1)