Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles

Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles Publisher Pubmed

Kordezangeneh M¹ ; Irani S¹ ; Mirfakhraie R² ; Esfandyarimanesh M³ ; Atyabi F^{3, 4} ; Dinarvand R^{3, 4}

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1. Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Nanotechnology Research Center, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
4. Department of Pharmaceutics, Tehran University of Medical Sciences, Tehran, Iran

Source: Medical Oncology Published:2015

Abstract

Today, using nanoparticle-based drug delivery systems has expanded to avoid anticancer side effects. Taxanes are important chemotherapeutic agents in the treatment of metastatic breast cancer. In this study, docetaxel (DTX)-loaded human serum albumin (HSA) nanoparticles (NPs) were prepared and characterized. Drug toxicity of the nanoparticles was measured by MTT assay with different drug concentrations (0.01, 0.1, 0.5, 1 and 5 μM) at different incubation times (24, 48 and 72 h). Expression of BAX/BCL2 mRNA levels was determined by real-time PCR. The size of NPs prepared and used in our study was about 147 nm with surface charge of −29.6 mV. Results obtained from MTT assay showed that 0.5 μM of free drug had 50 % toxicity on MCF-7 cells after 48-h incubation. Real-time PCR results showed an increase in expression of BAX and no change for BCL2. In conclusion, a significant overexpression of BAX gene and changes in BAX/BCL2 ratio were observed for DTX-loaded HSA nanoparticles compared with free DTX and may provide a potential therapy to inhibit anticancer drug resistance. © 2015, Springer Science+Business Media New York.

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Mohsen Amini (1)

Fatemeh Atyabi (1)

Style	Citing Format
MLA	Kordezangeneh M, et al.. "Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles." Medical Oncology, vol. 32, no. 7, 2015, pp. -.
APA	Kordezangeneh M, Irani S, Mirfakhraie R, Esfandyarimanesh M, Atyabi F, Dinarvand R (2015). Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles. Medical Oncology, 32(7), -.
Chicago	Kordezangeneh M, Irani S, Mirfakhraie R, Esfandyarimanesh M, Atyabi F, Dinarvand R. "Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles." Medical Oncology 32, no. 7 (2015): -.
Harvard	Kordezangeneh M et al. (2015) 'Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles', Medical Oncology, 32(7), pp. -.
Vancouver	Kordezangeneh M, Irani S, Mirfakhraie R, Esfandyarimanesh M, Atyabi F, Dinarvand R. Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles. Medical Oncology. 2015;32(7):-.
BibTex	@article{ author = {Kordezangeneh M and Irani S and Mirfakhraie R and Esfandyarimanesh M and Atyabi F and Dinarvand R}, title = {Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles}, journal = {Medical Oncology}, volume = {32}, number = {7}, pages = {-}, year = {2015} }
RIS	TY - JOUR AU - Kordezangeneh M AU - Irani S AU - Mirfakhraie R AU - Esfandyarimanesh M AU - Atyabi F AU - Dinarvand R TI - Regulation of Bax/Bcl2 Gene Expression in Breast Cancer Cells by Docetaxel-Loaded Human Serum Albumin Nanoparticles JO - Medical Oncology VL - 32 IS - 7 SP - EP - PY - 2015 ER -

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