Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Tracking of Gfp-Labeled Unrestricted Somatic Stem Cells Transplanted in the Sepsis Mouse Model Publisher Pubmed



Zareibehjani Z1 ; Soleimani M2, 3 ; Atashi A4 ; Ebrahimibarough S1 ; Ai J1 ; Hamidieh AA5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Hematology and Cell therapy department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Stem Cell Technology Research Center, Tehran, Iran
  4. 4. Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
  5. 5. Pediatric Stem Cell Transplant Department, Children's Medical center, Tehran University of Medical Sciences, Tehran, Iran

Source: Tissue and Cell Published:2019


Abstract

Cell-based therapy provides a promising approach for the treatment of sepsis and related disorders. Fate determination of transplanted cells is the most essential issue for cell therapist. Optical imaging is the reliable, time and cost-effective system for cell tracking. The present study was aimed to apply an optical imaging system for monitoring of GFP-labeled unrestricted somatic stem cells in the sepsis animal model. In vivo imaging showed the most accumulation of intravenously injected cells into the lungs and liver of septic mice. Thereafter, the imaging data were more approved by flow cytometry and immunohistochemistry staining. Cellular localization in septic lungs and liver that observed by optical imaging technique may offer beneficial evidence for designing of sepsis clinical trials. © 2019 Elsevier Ltd