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Osteoconduction of Unrestricted Somatic Stem Cells on an Electrospun Polylactic-Co-Glycolic Acid Scaffold Coated With Nanohydroxyapatite Publisher Pubmed



Shahi M1, 4 ; Nadari M5 ; Sahmani M2 ; Seyedjafari E6 ; Ahmadbeigi N5 ; Peymani A3
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
  2. 2. Department of Clinical Biochemistry and Medical Genetics, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
  3. 3. Medical Microbiology Research Center, Qazvin University of Medical Sciences, Bahonar Blvd Qazvin, Qazvin, 3419759811, Iran
  4. 4. SABZ Biomedicals Science-Based Company, Iran
  5. 5. Cell-Based Therapies Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Iran
  6. 6. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran

Source: Cells Tissues Organs Published:2018


Abstract

The limitation of traditional bone grafts could be overcome by applying engineered bone constructs, which are mainly produced by seeding suitable stem cells on appropriate scaffolds. So far, bone marrow-derived stromal cells have been the most applied cells in bone tissue engineering, but current data show that unrestricted somatic stem cells (USSCs) from human cord blood might actually be a better stem cell source due to the accessibility and noninvasive procedure of collection. In this study, we cultured USSCs on a plasma-treated electrospun polylactic-co-glycolic acid (PLGA) scaffold coated with nanohydroxyapatite (nHA). Adhesion and proliferation of USSCs on PLGA/nHA were assessed by scanning electron microscopy and MTT assay. Osteogenic differentiation of USSCs into osteoblast lineage cells was evaluated via alkaline phosphatase (ALP) activity and real-time polymerase chain reaction. Our observation showed that USSCs attached and proliferated on PLGA/nHA. Osteogenic differentiation was confirmed by increased ALP activity and OSTEONECTIN expression in USSCs on PLGA/nHA after the 1st week of the osteogenic period. Therefore, using USSCs on electrospun PLGA/nHA is a promising approach in bone tissue engineering. © 2018 S. Karger AG, Basel.
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