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Assessing the Physico-Mechanical, Anti-Bacterial, and Anti-Demineralization Properties of Orthodontic Resin Composite Containing Different Concentrations of Photoactivated Zinc Oxide Nanoparticles on Streptococcus Mutans Biofilm Around Ceramic and Metal Orthodontic Brackets: An Ex Vivo Study Publisher Pubmed



Hemmati YB1 ; Bahrami R2 ; Pourhajibagher M3
Authors
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Authors Affiliations
  1. 1. Dental Sciences Research Center, Department of Orthodontics, School of Dentistry, Guilan University of Medical Sciences, Rasht, Iran
  2. 2. Department of Orthodontics, School of Dentistry, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: International Orthodontics Published:2024


Abstract

Background and purpose: The aim of this study was to evaluate the physico-mechanical, anti-bacterial, and anti-demineralization properties of orthodontic resin composite containing photoactivated zinc oxide nanoparticles (ZnONPs) on Streptococcus mutans biofilm around ceramic and metal brackets. Material and methods: Following the minimum inhibitory concentration (MIC) determination for ZnONPs, shear bond strength (SBS) was tested for composites containing different concentrations of ZnONPs. The chosen concentration was used to evaluate the microleakage, anti-bacterial, and anti-demineralization properties. Results: Adding 50 μg/mL of ZnONPs to the orthodontic composite did not negatively affect its physico-mechanical properties. ZnONPs (50 μg/mL)-mediated aPDT and 0.2% chlorhexidine significantly (P = 0.000) reduced S. mutans biofilms compared to the phosphate-buffered saline (PBS) groups (metal/PBS = 7.47 ± 0.7 × 106, and ceramic/PBS = 7.47 ± 0.7 × 106), with the lowest colony count observed in these groups (metal/chlorhexidine = 1.06 ± 0.4 × 105, ceramic/chlorhexidine = 1 ± 0.2 × 105, metal/ZnONPs-mediated aPDT = 1.33 ± 0.3 × 105, and ceramic/ZnONPs-mediated aPDT = 1.2 ± 0.3 × 105). Sodium fluoride varnish and ZnONPs-mediated aPDT showed the highest efficacy in anti-demineralization and significantly improving the enamel surface microhardness compared to the artificial saliva, especially in ceramic bracket groups (524.17 ± 42.78 N and 441.00 ± 29.48 N, 394.17 ± 46.83 N, P = 0.000, and P = 0.003, respectively). Conclusion: ZnONPs (50 μg/mL)-mediated aPDT effectively inhibited S. mutans biofilm and promoted anti-demineralization without adverse effects on the physico-mechanical properties of the composite resin. These results suggest the potential of this method in preventing white spot lesions during orthodontic treatment. © 2024 CEO
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