Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis

Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis Publisher Pubmed

Mirkalantari S¹ ; Zarnani AH^{2, 3} ; Nazari M⁴ ; Irajian GR¹ ; Amirmozafari N¹

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1. Iran University of Medical Sciences, Microbiology Department, Faculty of Medicine, Tehran, Iran
2. Tehran University of Medical Sciences, Dept. of Immunology, School of Public Health, Tehran, Iran
3. Iran University of Medical Sciences, Immunology Research Center, Tehran, Iran
4. Avicenna Research Institute, ACECR, Monoclonal Antibody Reaserch Center, Tehran, Iran

Source: Annals of Clinical Microbiology and Antimicrobials Published:2017

Abstract

Background: The numerous drawbacks of current serological tests for diagnosis of brucellosis which mainly results from cross reactivity with LPS from other gram-negative bacteria have generated an increasing interest to find more specific non-LPS antigens. Previous studies had indicated that Brucella VirB12 protein, a cell surface protein and component of type IV secretion system, induces antibody response during animal infection. However, this protein has not yet been tested as a serological diagnostic marker in human brucellosis. Methods: Recombinant VirB12 protein was prepared and evaluated the efficacy of it in an indirect enzyme-linked immunosorbent assay (ELISA) for brucellosis with sera collected from different region of Iran and the results were compared with a commercial ELISA kit. Results: Sera from human brucellosis patients strongly reacted to the purified recombinant VirB12. The sensitivity, specificity, accuracy, negative predictive value and positive predictive value of recombinant VirB12-based ELISA related to the commercial-ELISA method were 87.8, 94, 90, 80 and 96.6% respectively. Conclusions: We concluded that antigenic VirB12 have a property value that can be considered as a candidate for using in serodiagnostic tests for human brucellosis. © 2017 The Author(s).

Style	Citing Format
MLA	Mirkalantari S, et al.. "Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis." Annals of Clinical Microbiology and Antimicrobials, vol. 16, no. 1, 2017, pp. -.
APA	Mirkalantari S, Zarnani AH, Nazari M, Irajian GR, Amirmozafari N (2017). Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis. Annals of Clinical Microbiology and Antimicrobials, 16(1), -.
Chicago	Mirkalantari S, Zarnani AH, Nazari M, Irajian GR, Amirmozafari N. "Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis." Annals of Clinical Microbiology and Antimicrobials 16, no. 1 (2017): -.
Harvard	Mirkalantari S et al. (2017) 'Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis', Annals of Clinical Microbiology and Antimicrobials, 16(1), pp. -.
Vancouver	Mirkalantari S, Zarnani AH, Nazari M, Irajian GR, Amirmozafari N. Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis. Annals of Clinical Microbiology and Antimicrobials. 2017;16(1):-.
BibTex	@article{ author = {Mirkalantari S and Zarnani AH and Nazari M and Irajian GR and Amirmozafari N}, title = {Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis}, journal = {Annals of Clinical Microbiology and Antimicrobials}, volume = {16}, number = {1}, pages = {-}, year = {2017} }
RIS	TY - JOUR AU - Mirkalantari S AU - Zarnani AH AU - Nazari M AU - Irajian GR AU - Amirmozafari N TI - Brucella Melitensis Virb12 Recombinant Protein Is a Potential Marker for Serodiagnosis of Human Brucellosis JO - Annals of Clinical Microbiology and Antimicrobials VL - 16 IS - 1 SP - EP - PY - 2017 ER -

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