Evaluation of Fibroblast Attachment in Root Conditioning With Er, Cr:Ysgg Laser Versus Edta: A Sem Study Publisher Pubmed



Fekrazad R¹ ; Lotfi G² ; Harandi M³ ; Ayremlou S⁴ ; Kalhori KAM⁵
Source: Microscopy Research and Technique Published:2015

Abstract

The regeneration of periodontal support is a main concern in periodontal therapy. This study aims to investigate the efficacy of Er, Cr:YSGG laser and EDTA based conditioning in attachment of fibroblast on root surfaces. This in vitro study was conducted on 81 root plates (6 mm × 4 mm × 1 mm) prepared from 27 single-rooted human mature teeth. The samples were divided into three groups: (1) Er, Cr: YSGG laser conditioning with a G6 tip (2.78 μm, 0.75 W, pulse duration of 140 μs, repetition rate of 20 Hz) for 5-7 s; (2) EDTA conditioning (17%, pH: 8) for 1 min; and (3) the control group which were exposed neither to EDTA nor laser. The viability and proliferation rates assessments were performed using MTT assay on days 3 and 5. In addition, the level of cell attachment was studied using scanning electron microscopy. The data indicated Er, Cr:YSGG conditioning increased cell viability by lapse of time (from days 3-5), with significantly better cell attachment compared to the other groups on days 3 and 5 (P<0.05). In addition, increasing cell attachment in the EDTA conditioning group compared with the control group was statistically significant on day 5 but not on day 3 (P<0.05). In conclusion, Er, Cr:YSGG laser conditioning can promote enhance fibroblast attachment on dentinal root surfaces more than EDTA. Microsc. Res. Tech. 78:317-322, 2015. © 2015 Wiley Periodicals, Inc.