Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model

Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model Publisher Pubmed

Mirshafiey A¹ ; Hosseini S¹ ; Afraei S² ; Rastkari N³ ; Zavareh FT¹ ; Azizi G^{4, 5}

Show Affiliations

1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Cell and Molecular Biology, Kish International Campus, University of Tehran, Kish, Iran
3. Center for Air Pollution, IER, Tehran University of Medical Sciences, Tehran, Iran
4. Imam Hassan Mojtaba Hospital, Alborz University of Medical Sciences, Karaj, Iran
5. Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Current Drug Discovery Technologies Published:2016

Abstract

Background: G2013 molecule is a novel non-steroidal anti-inflammatory agent with immunosuppressive property, which was investigated on determinants relative to the oxidative stress in animal model. Materials and Methods: The Sprague-Dawley rats were used for evaluating properties of G2013 on some oxidative stress enzymes including: Myeloperoxidase (MPO), Glutathione peroxidase (GPX1), mitochondrial Superoxide dismutase (SOD2), Catalase (CAT), Glutathione S-Transferase (GST), and inducible nitric oxide synthase (iNOS) genes expression by Real Time PCR. The rats were sacrificed 3 months after daily oral administration of G2013. Moreover, Malondialdehyde (MDA), Carbonyl protein (PCO), the lipid and protein oxidation markers respectively and total anti-oxidant capacity (TAC) were tested in serum by biochemical analysis. Also cortisol as a steroid hormone was evaluated by chemiluminescence immunoassay after 12 weeks consumption of G2013 solution. Result: Our findings revealed a significant decrease in MPO in G2013 treated group, indicating its favorable effects but has no significant effects on genes expression of another antioxidant enzymes, including: SOD2, CAT, GPX1, and GST. Also, there were no significant differences in PCO, TAC and cortisol compared to control group following G2013 consumption. While an enhancement in serum MDA level was observed in the treatment group. In addition, G2013 therapy did not show any weight loss. Conclusions: Our data showed the safety and efficacy of G2013 as a novel designed NSAID on various oxidative stress determinants. © 2016 Bentham Science Publishers.

2. An in Vitro Evaluation of Anti-Aging Effect of Guluronic Acid (G2013) Based on Enzymatic Oxidative Stress Gene Expression Using Healthy Individuals Pbmcs, Biomedicine and Pharmacotherapy (2017)

3. Effect of Β-D-Mannuronic Acid (M2000) on Oxidative Stress Enzymes’ Gene Using Healthy Donor Peripheral Blood Mononuclear Cells for Evaluating the Anti-Aging Property, Current Drug Discovery Technologies (2019)

4. Evaluation of the Effect of Α-L-Guluronic Acid (G2013) on Cox-1, Cox-2 Activity and Gene Expression for Introducing This Drug As a Novel Nsaid With Immunomodulatory Property, Recent Patents on Inflammation and Allergy Drug Discovery (2018)

5. Preclinical and Pharmacotoxicology Evaluation of Α-L-Guluronic Acid (G2013) As a Non-Steroidal Anti-Inflammatory Drug With Immunomodulatory Property, Immunopharmacology and Immunotoxicology (2017)

6. Therapeutic Effects of D-Aspartate in a Mouse Model of Multiple Sclerosis, Journal of Food and Drug Analysis (2017)

Experts (# of related papers)

Mohammad Abdollahi (2)

Noushin Rastkari (1)

Style	Citing Format
MLA	Mirshafiey A, et al.. "Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model." Current Drug Discovery Technologies, vol. 13, no. 1, 2016, pp. 25-33.
APA	Mirshafiey A, Hosseini S, Afraei S, Rastkari N, Zavareh FT, Azizi G (2016). Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model. Current Drug Discovery Technologies, 13(1), 25-33.
Chicago	Mirshafiey A, Hosseini S, Afraei S, Rastkari N, Zavareh FT, Azizi G. "Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model." Current Drug Discovery Technologies 13, no. 1 (2016): 25-33.
Harvard	Mirshafiey A et al. (2016) 'Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model', Current Drug Discovery Technologies, 13(1), pp. 25-33.
Vancouver	Mirshafiey A, Hosseini S, Afraei S, Rastkari N, Zavareh FT, Azizi G. Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model. Current Drug Discovery Technologies. 2016;13(1):25-33.
BibTex	@article{ author = {Mirshafiey A and Hosseini S and Afraei S and Rastkari N and Zavareh FT and Azizi G}, title = {Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model}, journal = {Current Drug Discovery Technologies}, volume = {13}, number = {1}, pages = {25-33}, year = {2016} }
RIS	TY - JOUR AU - Mirshafiey A AU - Hosseini S AU - Afraei S AU - Rastkari N AU - Zavareh FT AU - Azizi G TI - Anti-Aging Property of G2013 Molecule As a Novel Immunosuppressive Agent on Enzymatic and Non-Enzymatic Oxidative Stress Determinants in Rat Model JO - Current Drug Discovery Technologies VL - 13 IS - 1 SP - 25 EP - 33 PY - 2016 ER -

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