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The Effect of Casein Phosphopeptide-Amorphous Calcium Phosphate Containing Bonding Agents on Dentin Shear Bond Strength and Remineralization Potential: An in Vitro Study Publisher



Yeganeh PK1 ; Teimourian H2 ; Omidi BR3 ; Amiri F4 ; Tahriri M5 ; Farahmandpour N2
Authors
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Authors Affiliations
  1. 1. Department of Operative Dentistry, School of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran
  2. 2. Department of Operative Dentistry, Faculty of Dentistry, Kermanshah University of Medical Sciences, Kermanshah, Iran
  3. 3. Department of Restorative Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran
  4. 4. School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Dental Research Center, School of Dentistry, Marquette University, Milwaukee, WI, United States

Source: Pesquisa Brasileira em Odontopediatria e Clinica Integrada Published:2024


Abstract

Objective: To assess the effect of Casein Phosphopeptide-Amorphous Calcium Phosphate (ACP) containing bonding agents on dentin shear bond strength and remineralization potential. Material and Methods: This in vitro study evaluated 45 extracted human premolars. The teeth were decoronated, and the tooth crown was split into buccal and lingual halves. The specimens were then flat-grounded by a 180-grit abrasive. The specimens were then randomized into three groups (n=15). Adper Scotchbond Multi-Purpose (SBMP) primer and adhesive were used for bonding in the control group. ACP in 10wt% and 20wt% concentrations was added to SBMP adhesive and used in groups 2 and 3, respectively. After the application of primer and adhesive and light-curing them for 10 s, a transparent silicon cylinder was placed on a dentin surface and cured for 10 s; then, the cylinder was filled with composite resin and was cured for the 40s from each side. The specimens underwent 3000 thermal cycles, and a universal testing machine measured the SBS. To assess the remineralization quality, a total of 6 dentin samples (2 specimens for group) were prepared and underwent X-ray diffraction, attenuated total reflection Fourier-transform infrared spectroscopy, and scanning electron microscopy-energy dispersive X-ray analysis. One-way analysis of variance was used to analyze the data. The level of p<0.05 was considered significant. Results: No significant difference in dentin shear bond strength was noted between the groups (p>0.05) — the addition of ACP to SBMP adhesive enhanced dentin remineralization. Increasing the ACP concentration from 10% to 20% increased the formation of hydroxyapatite. Conclusion: Adding amorphous calcium phosphate confers remineralizing property to SBMP adhesive without compromising its shear bond strength to dentin. © 2024, Association of Support to Oral Health Research (APESB). All rights reserved.
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