Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Stable Expression of Anti-Cd52 Monoclonal Antibody Using a Bicistronic Vector System Publisher



Rahimpour A1, 2 ; Bayat H1, 2 ; Omidi M1, 2 ; Peyrovan M3 ; Mohammadian O4 ; Naderi F5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Medical Nano-Technology and oTissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Deaprtment of Cell and Molecular Biology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  4. 4. Departments of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Genetics, School of Advanced Sciences, Islamic Azad University, Tehran Medical Branch, Tehran, Iran

Source: Biology and Medicine Published:2016


Abstract

The efficient development of stable monoclonal antibody-producing mammalian cells is a tedious and time-consuming process due to the structural complexity of these molecules. The ratio of the light-chain to heavy-chain expression is critical for the assembly and successful production of functional antibodies. Different vector-design strategies have been employed for the optimal expression of monoclonal antibodies in mammalian cells. In the current study, a bicistronic expression based on the encephalomyocarditis virus internal ribosomal entry site (ECMV IRES) element was used for the development of Chinese hamster ovary (CHO)-stable cell pools expressing an anti-CD52 antibody. The success- ful expression of the monoclonal antibody in CHO cells was achieved with the maximum titer of 20 μg/l. Our results here show that IRES-mediated bicistronic expression is an efficient method for the stable expression of monoclonal antibodies in CHO cells. © 2016 Bayat et al.
Related Docs
1. Evaluation of Chemical Chaperones on the Transient Expression in Chinese Hamster, Journal of Global Pharma Technology (2015)
2. Evaluation of Different Vector Design Strategies for the Expression of Recombinant Monoclonal Antibody in Cho Cells, Preparative Biochemistry and Biotechnology (2018)
3. The Augmenting Effects of the Tdna Insulator on Stable Expression of Monoclonal Antibody in Chinese Hamster Ovary Cells, Monoclonal Antibodies in Immunodiagnosis and Immunotherapy (2018)
4. Humanizing Glycosylation Pathways in Eukaryotic Expression Systems, World Journal of Microbiology and Biotechnology (2017)
5. Development of an Improved Lentiviral Based Vector System for the Stable Expression of Monoclonal Antibody in Cho Cells, Preparative Biochemistry and Biotechnology (2019)