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Evaluation of Chemical Chaperones on the Transient Expression in Chinese Hamster



Dadmarzi M1 ; Rahimpour A2 ; Bidoki SK3
Authors
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Authors Affiliations
  1. 1. Department of Genetics, School of Basic Sciences, Payame Noor University, Shahre Rey Branch, Ray, Tehran, Iran
  2. 2. Medical Nano-Technology and Tissue Engineering Research Center, Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Genetics and Biotechnology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran

Source: Journal of Global Pharma Technology Published:2015

Abstract

Recombinant proteins play an important role in biomedical and basic science researches. In this regard, mammalian cells have widely use in monoclonal antibodies. Improper aggregation complex recombinant proteins have been known as a bottleneck in successful production of active bio-therapeutics. Anti-CD52 is efficient immunosuppressive agents of therapeutic applications especially in autoimmune diseases. In this study, effects of four chemical Chaperones including DMSO and 4-phenyl butyrate (4-PBA) on transient of a recombinant evaluated during six days. Analysis of the expression level showed up to 2 fold in DMSO treated cells, while 4-PBA treatment improved antibody expression up to 50%, 30% and 10%, respectively. © 2009-2016, JGPT.
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