Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Assessment of the Photobiomodulation Effect of a Blue Diode Laser on the Proliferation and Migration of Cultured Human Gingival Fibroblast Cells: A Preliminary in Vitro Study Publisher



Etemadi A1, 2 ; Namin ST3 ; Hodjat M4 ; Kosarieh E2 ; Hakimiha N5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of periodontology, Faculty of Dentistry, Islamic Azad university, Tehran, Iran
  2. 2. Laser Research Center of Dentistry, Dental Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. DDS, Private practice, Tehran, Iran
  4. 4. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. DDS, PhD candidate in laser dentistry, Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Lasers in Medical Sciences Published:2020


Abstract

Introduction: Photobiomodulation therapy (PBM) is emerging as an effective strategy for the management of wound healing. The application of red and near infra-red light sources in laser therapy has been the subject of most researches in recent literature. Considering the lack of sufficient evidence in assessing the blue light in PBM, we aimed to investigate the photobiomodulation effect of a blue diode laser on the proliferation and migration of cultured human gingival fibroblast cells as a preliminary in vitro study. Methods: Human gingival fibroblast cells were irradiated with a blue diode laser at a 445 nm wavelength. Irradiation was done using three different powers of 200 mW (irradiation times of 5, 10,15, and 20 seconds); 300 mW (irradiation times of 5, 10, and 15 seconds); and 400 mW (irradiation times of 5 and 10 seconds). The fibroblast cells without laser exposure were considered as control. After 24 hours of incubation, the MTT assay and the wound scratch test were performed on the cells to investigate the biomodulation effect of the blue laser on the proliferation and migration of the cells respectively. The results were analyzed by one-way ANOVA and a post-hoc Tukey test with a P value <0.05 as a statistical significance level. Results: PBM with blue diode laser at power densities of 400 mW/cm2 with irradiation times of 10 and 15 seconds corresponding to energy densities of 4 and 6 J/cm2 exerted the statistically significant positive effect on both proliferation and migration of gingival fibroblast cells. Conclusion: Considering the encouraging findings of this study, PBM with blue diode laser can promote proliferation and migration of human gingival fibroblasts, the key cells involved in the process of oral wound healing. © 2020. Journal of Lasers in Medical Sciences. All Rights Reserved.
Related Docs
1. In Vitro Effect of 445 Nm Blue Laser and 660 Nm Low-Level Laser on the Quantity and Quality of Human Gingival Fibroblasts, Photochemistry and Photobiology (2023)
2. Photobiomodulation Effects of Pulsed and Continuous Wave Near-Infrared Laser on the Proliferation and Migration of Human Gingival Fibroblasts: An in Vitro Study, Photochemistry and Photobiology (2024)
3. Effect of Different Energy Densities of 915 Nm Low Power Laser on the Biological Behavior of Human Gingival Fibroblast Cells in Vitro, Photochemistry and Photobiology (2022)
4. Photobiomodulation Effect of Different Diode Wavelengths on the Proliferation of Human Gingival Fibroblast Cells, Photochemistry and Photobiology (2021)
5. Effects of Photobiomodulation and Split-Thickness Skin Grafting in the Prognosis of Wound Healing in Children With Deep Burn Ulcers, Photobiomodulation# Photomedicine# and Laser Surgery (2022)
6. Effect of Gaalas 940 Nm Photobiomodulation on Palatal Wound Healing After Free Gingival Graft Surgery: A Split Mouth Randomized Controlled Clinical Trial, BMC Oral Health (2022)
Experts (# of related papers)
View all Related Experts
Mohammad Ali Nilforoushzadeh (1)
Mahshid Hodjat (1)