Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model

Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model Publisher Pubmed

Bakht Azad S¹ ; Nikokar I^{1, 2} ; Faezi S² ; Rasooly S¹ ; Mahdavi M^{3, 4}

Show Affiliations

1. Medical Biotechnology Research Center, Laboratory of Microbiology and Immunology of Infectious Diseases, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran
2. Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran
3. Recombinant Vaccine Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran

Source: Microbial Pathogenesis Published:2018

Abstract

Considering the increased antibiotic resistance of Pseudomonas aeruginosa, the evaluation of immune response against the antigens of this bacterium seems necessary. In this study, the protective efficacy and immunological properties of P. aeruginosa recombinant PilQ (r-PilQ) and type b-flagellin (FLB) proteins was evaluated in the burn mouse model of infection. The inbred BALB/c mice were immunized with r-PilQ and FLB antigens. To investigate the type of induced immune response, sera were analyzed by ELISA for total IgG, IgG1, and IgG2a isotypes. After the final immunization, the IL-4, IFN-γ and IL-17 cytokines level were examined in the spleen of non-challenged mice. Fifty days after lethal challenge, the survival rate and bacterial burden in the skin and other internal organs of experimental mice were assessed. The in vivo administration of r-PilQ, FLB and combined antigen resulted in a significant increase in the survival of mice (66%, 75%, and 83%, respectively) infected by the PAO1 strain of P. aeruginosa in the burn model of infection. Immunization of mice with r-PilQ and FLB mixture induced high titers of IL-4 and IL-17 cytokines compared to control groups (P < 0.05). The high titer of antisera raised against combined antigen was able to inhibit the systemic spread of the PAO1 strain from the site of infection to the internal organs. We concluded that the parallel role of IL-4 and IL-17 is necessary for elimination of the bacteria and promotion of survival in the immunized burn mice. © 2018

Related Docs

View other Related Docs

1. A Trivalent Vaccine Consisting of “Flagellin A+B and Pilin” Protects Against Pseudomonas Aeruginosa Infection in a Murine Burn Model, Microbial Pathogenesis (2020)

2. Immunization With Bivalent Flagellin Protects Mice Against Fatal Pseudomonas Aeruginosa Pneumonia, Journal of Immunology Research (2017)

3. Cloning, Expression, and Purification of Pseudomonas Aeruginosa Flagellin, and Characterization of the Elicited Anti-Flagellin Antibody, Iranian Red Crescent Medical Journal (2016)

4. Flagellin and Pilin Immunization Against Multi-Drug Resistant Pseudomonas Aeruginosa Protects Mice in the Burn Wound Sepsis Model, Immunology Letters (2016)

5. Immunization With Recombinant Hpv16-E7d in Fusion With Flagellin As a Cancer Vaccine: Effect of Antigen-Adjuvant Orientation on the Immune Response Pattern, Immunologic Research (2025)

6. Immunogenicity and Protective Efficacy of Pseudomonas Aeruginosa Type a and B Flagellin Vaccines in a Burned Mouse Model, Molecular Immunology (2016)

7. Nanomedicine and Advanced Technologies for Burns: Preventing Infection and Facilitating Wound Healing, Advanced Drug Delivery Reviews (2018)

8. Expression of Salmonella Typhimurium and Escherichia Coli Flagellin Protein and Its Functional Characterization As an Adjuvant, Microbial Pathogenesis (2018)

Experts (# of related papers)

View all Related Experts

Farhad Bonakdar Hashmi (5)

Mehdi Shafee Ardestani (1)

Style	Citing Format
MLA	Bakht Azad S, et al.. "Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model." Microbial Pathogenesis, vol. 123, no. , 2018, pp. 426-432.
APA	Bakht Azad S, Nikokar I, Faezi S, Rasooly S, Mahdavi M (2018). Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model. Microbial Pathogenesis, 123(), 426-432.
Chicago	Bakht Azad S, Nikokar I, Faezi S, Rasooly S, Mahdavi M. "Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model." Microbial Pathogenesis 123, no. (2018): 426-432.
Harvard	Bakht Azad S et al. (2018) 'Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model', Microbial Pathogenesis, 123(), pp. 426-432.
Vancouver	Bakht Azad S, Nikokar I, Faezi S, Rasooly S, Mahdavi M. Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model. Microbial Pathogenesis. 2018;123():426-432.
BibTex	@article{ author = {Bakht Azad S and Nikokar I and Faezi S and Rasooly S and Mahdavi M}, title = {Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model}, journal = {Microbial Pathogenesis}, volume = {123}, number = {}, pages = {426-432}, year = {2018} }
RIS	TY - JOUR AU - Bakht Azad S AU - Nikokar I AU - Faezi S AU - Rasooly S AU - Mahdavi M TI - Evaluation of the Immune Responses Following Co-Administration of Pilq and Type B-Flagellin From Pseudomonas Aeruginosa in the Burn Mouse Model JO - Microbial Pathogenesis VL - 123 IS - SP - 426 EP - 432 PY - 2018 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract