Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells

Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells Publisher Pubmed

Yarahmadi S¹ ; Abdolvahabi Z² ; Hesari Z^{3, 4} ; Tavakoliyaraki M¹ ; Yousefi Z¹ ; Seiri P⁵ ; Hosseinkhani S⁶ ; Nourbakhsh M^{1, 7}

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1. Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Department of Biochemistry and Genetics, Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
3. Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran
4. Department of Laboratory Science, Faculty of Paramedicine, Golestan University of Medical Sciences, Gorgan, Iran
5. Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
7. Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran

Source: Gene Published:2019

Abstract

Sirtuin 1 is one of the regulators of cell growth and survival and its inhibition is suggested as a suitable mechanism to overcome breast cancer development. In this study we explored the role of miR-211-5p in SIRT1/p53 pathway and its influence on breast cancer cell viability and apoptosis. Cells were transfected with miR-211-5p mimic and inhibitor to modulate cellular miR-211-5p levels in breast cancer cell lines, MDA-MB-231 and MCF-7. Gene expression of miR-211-5p and SIRT1 were measured with real-time PCR. SIRT1 protein level and the acetylation of p53 as well as SIRT1 activity were evaluated by Western blotting and fluorometry, respectively. In order to explore the direct attachment of miR-211-5p to the 3′-UTR of SIRT1 mRNA, luciferase reporter assay was applied. Cell viability in response to miR-211-5p was studied by MTT assay and apoptosis was assessed by annexin V labeling followed by flow cytometry. Results showed that SIRT1 gene and protein expression were inhibited by miR-211-5p and the 3′-UTR of SIRT1 was found to be directly targeted by miR-211-5p. Inhibition of SIRT1 expression resulted in its reduced activity. Up-regulation of miR-211-5p was also followed by a significant decline in the acetylation status of p53 which was associated with remarkable decreased cell viability and induction of apoptosis in breast cancer cells. Antisense oligonucleotide of miR-211-5p acted as its inhibitor and exerted opposite effects both on SIRT1 expression and cell apoptosis. In conclusion, inhibition of SIRT1 by miR-211-5p could effectively reduce breast cancer cell survival and cause cell death and therefore might be considered a seemly mechanism for designing anticancer therapies. © 2019 Elsevier B.V.

2. Microrna-340 Inhibits the Migration, Invasion, and Metastasis of Breast Cancer Cells by Targeting Wnt Pathway, Tumor Biology (2016)

3. Mir-130A-3P Blocks Wnt Signaling Cascade in the Triple-Negative Breast Cancer by Targeting the Key Players at Multiple Points, Heliyon (2020)

4. Extracellular Nampt/Visfatin Causes P53 Deacetylation Via Nad Production and Sirt1 Activation in Breast Cancer Cells, Cell Biochemistry and Function (2017)

5. Beneficial Effects of Omega-3 and Vitamin E Coadministration on Gene Expression of Sirt1 and Pgc1α and Serum Antioxidant Enzymes in Patients With Coronary Artery Disease, Nutrition# Metabolism and Cardiovascular Diseases (2016)

6. Competing Endogenous Rna (Cerna) Cross Talk and Language in Cerna Regulatory Networks: A New Look at Hallmarks of Breast Cancer, Journal of Cellular Physiology (2019)

7. The Role of Noncoding Rnas in Pituitary Adenoma, Epigenomics (2021)

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Style	Citing Format
MLA	Yarahmadi S, et al.. "Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells." Gene, vol. 711, no. , 2019, pp. -.
APA	Yarahmadi S, Abdolvahabi Z, Hesari Z, Tavakoliyaraki M, Yousefi Z, Seiri P, Hosseinkhani S, Nourbakhsh M (2019). Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells. Gene, 711(), -.
Chicago	Yarahmadi S, Abdolvahabi Z, Hesari Z, Tavakoliyaraki M, Yousefi Z, Seiri P, Hosseinkhani S, Nourbakhsh M. "Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells." Gene 711, no. (2019): -.
Harvard	Yarahmadi S et al. (2019) 'Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells', Gene, 711(), pp. -.
Vancouver	Yarahmadi S, Abdolvahabi Z, Hesari Z, Tavakoliyaraki M, Yousefi Z, Seiri P, et al.. Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells. Gene. 2019;711():-.
BibTex	@article{ author = {Yarahmadi S and Abdolvahabi Z and Hesari Z and Tavakoliyaraki M and Yousefi Z and Seiri P and Hosseinkhani S and Nourbakhsh M}, title = {Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells}, journal = {Gene}, volume = {711}, number = {}, pages = {-}, year = {2019} }
RIS	TY - JOUR AU - Yarahmadi S AU - Abdolvahabi Z AU - Hesari Z AU - Tavakoliyaraki M AU - Yousefi Z AU - Seiri P AU - Hosseinkhani S AU - Nourbakhsh M TI - Inhibition of Sirtuin 1 Deacetylase by Mir-211-5P Provides a Mechanism for the Induction of Cell Death in Breast Cancer Cells JO - Gene VL - 711 IS - SP - EP - PY - 2019 ER -

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