Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice

Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice Publisher Pubmed

Ahmadi H¹ ; Fathi F² ; Karimi H¹ ; Amidi F¹ ; Mehdinejadiani S¹ ; Moeini A³ ; Bahram Rezai M¹ ; Hoseini S⁴ ; Sobhani A¹

Show Affiliations

1. Department of Anatomy, Medical School, Tehran University of Medical Sciences, Tehran, Iran
2. Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3. Department of Obstetrics and Gynecology, Arash Women's Hospital, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Anatomy, School of Medicine, International Campus, Tehran University of Medical Sciences (IC-TUMS), Tehran, Iran

Source: Journal of Reproductive Immunology Published:2020

Abstract

Aim and purpose: The higher prevalence rate of different diseases may accentuate the possible alteration of the immune system in individuals conceived through the assisted reproductive technologies (ART). The present study was conducted to evaluate the TH1, TH2, TH17 balance in intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) - conceived mice in comparison to naturally conceived offspring. Methods: Mice (6–8 weeks) were divided into three groups (IVF- conceived, ICSI- conceived and naturally conceived). They were subjected to subcutaneous immunization witMycobacterium bovis Bacille Calmette-Guerin (BCG). The blood samples were taken and the sera were separated. Then the spleens were surgically removed at the time the mice were sacrificed. Serum levels of IFN-γ, IL-17A and IL-4 were detected by ELISA. Then the proportion of TCD4 cells possessing the T-bet+, GATA3+, and ROR-γt + were measured using FACS caliber flow cytometer. Results: In comparison with naturally conceived mice, intracellular expression of T-bet and serum levels of IFN-γ were significantly decreased in ART- conceived mice. Moreover, ART- conceived offspring showed marked increase in IL-4 and IL-17A. Conclusion: It is concluded that compared to naturally conceived male mice, ART- conceived counterparts exhibit less efficient immune responses against BCG through further promotion of humoral and inflammatory related immune response characteristics. © 2020

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Style	Citing Format
MLA	Ahmadi H, et al.. "Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice." Journal of Reproductive Immunology, vol. 139, no. , 2020, pp. -.
APA	Ahmadi H, Fathi F, Karimi H, Amidi F, Mehdinejadiani S, Moeini A, Bahram Rezai M, Hoseini S, Sobhani A (2020). Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice. Journal of Reproductive Immunology, 139(), -.
Chicago	Ahmadi H, Fathi F, Karimi H, Amidi F, Mehdinejadiani S, Moeini A, Bahram Rezai M, Hoseini S, Sobhani A. "Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice." Journal of Reproductive Immunology 139, no. (2020): -.
Harvard	Ahmadi H et al. (2020) 'Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice', Journal of Reproductive Immunology, 139(), pp. -.
Vancouver	Ahmadi H, Fathi F, Karimi H, Amidi F, Mehdinejadiani S, Moeini A, et al.. Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice. Journal of Reproductive Immunology. 2020;139():-.
BibTex	@article{ author = {Ahmadi H and Fathi F and Karimi H and Amidi F and Mehdinejadiani S and Moeini A and Bahram Rezai M and Hoseini S and Sobhani A}, title = {Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice}, journal = {Journal of Reproductive Immunology}, volume = {139}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Ahmadi H AU - Fathi F AU - Karimi H AU - Amidi F AU - Mehdinejadiani S AU - Moeini A AU - Bahram Rezai M AU - Hoseini S AU - Sobhani A TI - Altered Th1, Th2, Th17 Balance in Assisted Reproductive Technology Conceived Mice JO - Journal of Reproductive Immunology VL - 139 IS - SP - EP - PY - 2020 ER -

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