Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust

Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust Publisher

Gholami A¹ ; Golbabaei F² ; Teimori G³ ; Kianmehr M⁴ ; Yaseri M⁵

Show Affiliations

1. Department of Occupational Health Engineering, School of Public Health, Birjand University of Medical Sciences, Birjand, Iran
2. Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Environmental Health, School of Health, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
4. Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
5. Department of Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Open Biochemistry Journal Published:2019

Abstract

Background and Objectives: Occupational exposure to silica dust can lead to biochemical damage. Malondialdehyde (MDA) can be considered as a primary marker for measuring the level of oxidative stress in a living organism. This study was conducted in order to evaluate the level of MDA in blood and urine of mice exposed to silica dust. Material and Methods: In this experimental study, 72 mice (BALB/c) were randomly allocated to five exposed groups and 1 control group. Exposure of mice to pure 99% silica dust was done in closed containers. Blood sampling was performed from the heart of mice and urine sampling fulfilled by insertion into a metabolic cage. The RAO et al. method was used to measure MDA. Results: The highest level of plasma MDA in group 1 in the 4th month was 8.4±0.41 nmol/l and the lowest level of MDA was 1.3±0.2 nmol/l in the third sampling in the control group, also the highest amount of urine MDA in the first and second groups and 4 months after exposure was 1.16±0.51 nmol/l, and the lowest in the control group and in the third sampling was 0.48±0.06 nmol/l. A significant difference was found between the levels of MDA in all exposed groups at different times except for the 5th group with the lowest concentration (P < 0.05). Conclusion: MDA in blood and urine could be proposed as a good biomarker for the evaluation of biochemical damages caused by silica dust. Measuring MDA is also a simple and inexpensive method that does not require complex equipment and can be used as an early detection test for biochemical damages caused by silica. © 2019 Gholami et al.

Style	Citing Format
MLA	Gholami A, et al.. "Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust." Open Biochemistry Journal, vol. 13, no. 1, 2019, pp. 32-36.
APA	Gholami A, Golbabaei F, Teimori G, Kianmehr M, Yaseri M (2019). Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust. Open Biochemistry Journal, 13(1), 32-36.
Chicago	Gholami A, Golbabaei F, Teimori G, Kianmehr M, Yaseri M. "Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust." Open Biochemistry Journal 13, no. 1 (2019): 32-36.
Harvard	Gholami A et al. (2019) 'Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust', Open Biochemistry Journal, 13(1), pp. 32-36.
Vancouver	Gholami A, Golbabaei F, Teimori G, Kianmehr M, Yaseri M. Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust. Open Biochemistry Journal. 2019;13(1):32-36.
BibTex	@article{ author = {Gholami A and Golbabaei F and Teimori G and Kianmehr M and Yaseri M}, title = {Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust}, journal = {Open Biochemistry Journal}, volume = {13}, number = {1}, pages = {32-36}, year = {2019} }
RIS	TY - JOUR AU - Gholami A AU - Golbabaei F AU - Teimori G AU - Kianmehr M AU - Yaseri M TI - Investigation of Blood and Urine Malondialdehyde Levels in Mice Exposed to Silica Dust JO - Open Biochemistry Journal VL - 13 IS - 1 SP - 32 EP - 36 PY - 2019 ER -

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