Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene

Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene Publisher Pubmed

Ahmadi B^{1, 2} ; Mirhendi H³ ; Makimura K⁴ ; De Hoog GS⁵ ; Shidfar MR² ; Nouripoursisakht S⁶ ; Jalalizand N²

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1. Department of Microbiology and Parasitology, School of Para-Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
2. Departments of Medical Parasitology & Mycology, School of Public Health, National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran
3. Departments of Medical Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4. Teikyo University Institute of Medical Mycology and Genome Research Center, Tokyo, Japan
5. Fungal Biodiversity Center, Institute of the Royal Netherlands, Academy of Arts and Sciences, Centraalbureau voor Schimmelcultures-KNAW, Utrecht, Netherlands
6. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

Source: Medical Mycology Published:2016

Abstract

Use of phylogenetic species concepts based on rDNA internal transcribe spacer (ITS) regions have improved the taxonomy of dermatophyte species; however, confirmation and refinement using other genes are needed. Since the calmodulin gene has not been systematically used in dermatophyte taxonomy, we evaluated its intra- and interspecies sequence variation as well as its application in identification, phylogenetic analysis, and taxonomy of 202 strains of 29 dermatophyte species. A set of primers was designed and optimized to amplify the target followed by bilateral sequencing. Using pairwise nucleotide comparisons, a mean similarity of 81% was observed among 29 dermatophyte species, with inter-species diversity ranging from 0 to 200 nucleotides (nt). Intraspecies nt differences were found within strains of Trichophyton interdigitale, Arthroderma simii, T. rubrum and A. vanbreuseghemii, while T. tonsurans, T. violaceum, Epidermophyton floccosum, Microsporum canis, M. audouinii, M. cookei, M. racemosum, M. gypseum, T. mentagrophytes, T schoenleinii, and A. benhamiae were conserved. Strains of E. floccosum/M. racemosum/M. cookei, A. obtosum/A. gertleri, T. tonsurans/T. equinum and a genotype of T. interdigitale had identical calmodulin sequences. For the majority of the species, tree topology obtained for calmodulin gene showed a congruence with coding and non-coding regions including ITS, BT2, and Tef-1I±. Compared with the phylogenetic tree derived from ITS, BT2, and Tef-1I± genes, some species such as E. floccosum and A. gertleri took relatively remote positions. Here, characterization and obtained dendrogram of calmodulin gene on a broad range of dermatophyte species provide a basis for further discovery of relationships between species. Studies of other loci are necessary to confirm the results. © 2016, A© The Author 2016. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com

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Style	Citing Format
MLA	Ahmadi B, et al.. "Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene." Medical Mycology, vol. 54, no. 5, 2016, pp. 500-514.
APA	Ahmadi B, Mirhendi H, Makimura K, De Hoog GS, Shidfar MR, Nouripoursisakht S, Jalalizand N (2016). Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene. Medical Mycology, 54(5), 500-514.
Chicago	Ahmadi B, Mirhendi H, Makimura K, De Hoog GS, Shidfar MR, Nouripoursisakht S, Jalalizand N. "Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene." Medical Mycology 54, no. 5 (2016): 500-514.
Harvard	Ahmadi B et al. (2016) 'Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene', Medical Mycology, 54(5), pp. 500-514.
Vancouver	Ahmadi B, Mirhendi H, Makimura K, De Hoog GS, Shidfar MR, Nouripoursisakht S, et al.. Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene. Medical Mycology. 2016;54(5):500-514.
BibTex	@article{ author = {Ahmadi B and Mirhendi H and Makimura K and De Hoog GS and Shidfar MR and Nouripoursisakht S and Jalalizand N}, title = {Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene}, journal = {Medical Mycology}, volume = {54}, number = {5}, pages = {500-514}, year = {2016} }
RIS	TY - JOUR AU - Ahmadi B AU - Mirhendi H AU - Makimura K AU - De Hoog GS AU - Shidfar MR AU - Nouripoursisakht S AU - Jalalizand N TI - Phylogenetic Analysis of Dermatophyte Species Using Dna Sequence Polymorphism in Calmodulin Gene JO - Medical Mycology VL - 54 IS - 5 SP - 500 EP - 514 PY - 2016 ER -

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