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The Effect of Prenatal Exposure to 2.4 Ghz Radio Frequency on the Histology and Expression of the Osteocalcin and Runx2 Gene of the Forelimb in an Nmri Mouse Publisher



Saghezchi SA1 ; Azad N2 ; Heidari R3 ; Jajarmi V4 ; Abdi S5 ; Abaszadeh HA6 ; Sadjadpour SS7 ; Neikoei N8 ; Heidari MH6 ; Abdollahifar MA6
Authors
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Authors Affiliations
  1. 1. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Abnormal Uterine Bleeding Research Center, Semnan University of Medical Sciences, Semnan, Iran
  3. 3. Otorhinolaryngology Research Center, Occupational Sleep Research Center, Department of Otorhinolaryngology, Imam Khomeini Educational Complex Hospital, Valiasr Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Anatomical Sciences and Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  6. 6. Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Department of Cell and Molecular Biology, School of biology, College of Science, University of Tehran, Iran
  8. 8. Information Technology Management department, Islamic Azad University Electronic Unit, Tehran, Iran

Source: Journal of Lasers in Medical Sciences Published:2019


Abstract

Introduction: Today the use of electromagnetic waves has dramatically increased in modern industrial societies. This study aimed to investigate the effect of prenatal exposure to 2.4 GHz wireless frequency on forelimb development in an NMRI mouse in vivo. Methods: A total of 21 female mice weighing 25-30 g were included in the present study. They were randomly assigned to 3 groups, namely control (n=7), sham (n=7), and experimental (n=7). After mating, the experimental group was exposed to 2.4 GHz radio frequency at a distance of 20-30 cm from the device, 4 h/d until the delivery. The sham group was placed at a distance of 20-30 cm from the device every day without exposure to electromagnetic waves, and the control group had a pregnancy period without any stress and electromagnetic wave exposure. After giving birth, the forelimbs were isolated from the infants and examined by stereological studies and RT-PCR for the evaluation of osteocalcin and RUNX2 gene expression. Results: Although, at first glance, there was no macroscopic teratogen effect in forelimbs in all groups, via a stereological method, we showed that bone and cartilage volume decreased in the experimental group compared to the other groups. We also found that the experimental group had lower expression of the osteocalcin and RUNX2 gene than the control and sham groups did. However, there were no significant differences between the control and sham groups in terms of bone and cartilage volume and gene expression. Conclusion: Although teratogen effect of prenatal exposure to 2.4 GHz radio frequency on forelimbs was not demonstrated macroscopically, further studies showed negative effects on the forelimb bone, cartilage volume, and gene expression. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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