Optimization of Metabolic Intermediates to Enhance the Production of Fucoxanthin From Tisochrysis Lutea Publisher



Mohamadnia S^{1, 2} ; Tavakoli O¹ ; Faramarzi MA² Show Affiliations
Source: Journal of Applied Phycology Published:2022

Abstract

A statistical experimental design using response surface methodology (RSM) was carried out to optimize the mixotrophic growth using glutamic acid, tri-sodium citrate, succinic acid, sodium aspartate, and sodium pyruvate to improve the production of fucoxanthin. First, a fractional factorial design as a screening test was done with the above-mentioned intermediates. Then, glutamic acid, sodium aspartate, and sodium pyruvate were chosen according to their statistically significant (P<0.05) and positive effects on fucoxanthin production. The three selected metabolic intermediates were optimized employing RSM to obtain a high level of fucoxanthin. The data were adjusted with a second-order polynomial equation to determine the relationship between fucoxanthin production and three optimized metabolic intermediates. Using multiple regression techniques and to attain the high fucoxanthin productivity (22.4 mg L−1 day−1), the optimum amount of the metabolic intermediates were found as follows: sodium aspartate, 7.5 mM; sodium pyruvate, 7.5 mM; glutamic acid, 3.29 mM. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.