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Repeated Daily Normobaric Hyperoxia: A Non-Pharmacological Strategy Against Gentamicin-Induced Nephrotoxicity



Akbari Z1 ; Ansari I2 ; Karimi Z3 ; Zendeboodi S4 ; Tanha K5 ; Asadi M6 ; Pourkhalili KH1
Authors
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Authors Affiliations
  1. 1. Department of Physiology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  2. 2. Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran
  3. 3. Nephro-Urology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Department of Internal Medicine, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  5. 5. Medical Physics and Biomedical Engineering Department, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. The Persian Gulf Nuclear Medicine Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran

Source: Iranian South Medical Journal Published:2023

Abstract

Background: Gentamicin (GM) induced nephrotoxicity is one of the most common causes of acute kidney injury and limits its administration. Evidence suggests that pre-treatment with oxygen increases the activity of antioxidant enzymes and protects tissues against different kinds of damage. In this study, the effect of daily normobaric heperoxia pre-treatment on alleviating gentamicin induced nephrotoxicity was investigated. Materials and Methods: Twenty-eight male Wistar rats were randomly divided into four groups (n=7): Control (room air); Gentamicin (100 mg/kg, IP, for 9 days); Hyperoxia60 (60 min daily pretreatment with 95% oxygen and then 100 mg/kg gentamicin, IP, for 9 days) and Hyperoxia180 (180 min daily pretreatment with 95% oxygen and then 100 mg/kg gentamicin, IP, for 9 days). Then 24 h urine was collected and on day 10, the rats were sacrificed for serum, urine and renal tissue sampling. Results: Results showed that hyperoxia significantly enhanced renal antioxidative capacity and decreased serum creatinine and renal failure index. Histological examination and SPECT scan also showed that tissue damage in the hyperoxia groups was lower compared to the gentamicin group. However, hyperoxia effect on body weight, kidney-to-body weight ratio, urine volume, blood urea nitrogen and glomerular filtration was not significant. Conclusion: Although pre-treatment with hyperoxia enhances the antioxidant capacity of renal tissue and improves some of the functional and histopathological parameters of the kidney, it failed to completely restore the adverse effects of gentamicin-induced nephrotoxicity. Thus, more studies are needed to determine the clinical effect of hyperoxia on gentamicin-induced nephrotoxicity. ©Iran South Med J. All rights reserved.
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