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Validated Spectrofluorimetric and Hplc-Uv Method for Determination of Memantine in Pharmaceutical Dosage Forms



Souri E1 ; Sarab ZR1 ; Rahmani M1 ; Adib N2 ; Ahmadkhaniha R3
Authors
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Authors Affiliations
  1. 1. Department of Medicinal Chemistry, Faculty of Pharmacy, Drug Design and Development Research Center, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
  2. 2. Department of Pharmaceutics, Food and Drug Laboratory Research Center, Ministry of Health, Tehran, Iran
  3. 3. Department of Human Ecology, School of Public health, Tehran University of Medical Sciences, Tehran, 1417614411, Iran

Source: Research Journal of Pharmaceutical# Biological and Chemical Sciences Published:2015

Abstract

Two simple and accurate methods were developed for the determination of memantine in bulk drug and pharmaceutical dosage forms. Memantine was derivatized using fluorescamine in borate buffer at pH 9.5. The derivatization reaction was optimized and the reaction product was determined by HPLC-UV method at 306 nm or spectrofluorimetric method at 475 nm (excitation at 395 nm). The chromatographic separation was achieved on a Nova-Pak CN column using a mixture of acetonitrile and water containing 0.07% TEA and 0.1% H3PO4 (40:60) as mobile phase. The calibration curves were linear over the concentration range of 1-80 μg/mL and 4-100 μg/mL for HPLC-UV and spectrofluorimetric method, respectively. The within-day and between-day coefficient of variations were less than 2 and 3% for HPLC-UV and spectrofluorimetric method, respectively. The proposed methods were successfully used for the determination of memantine in pharmaceutical dosage forms which showed acceptable results without any interference from the excipients.