Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Glial Response to Intranasal Mesenchymal Stem Cells in Intermittent Cuprizone Model of Demyelination Publisher Pubmed



Zarini D1 ; Pasbakhsh P1 ; Shabani M2 ; Mojaverrostami S1 ; Hashemi M1 ; Amirizadeh S1 ; Majidpoor J3 ; Omidi A4 ; Mortezaee K5 ; Kashani IR1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Anatomy, School of Medicine, Infectious Diseases Research Center, Gonabad University of Medical Sciences, Gonabad, Iran
  4. 4. Department of Anatomical Science, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  5. 5. Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

Source: Neurotoxicity Research Published:2022


Abstract

Intranasal mesenchymal stem cells (MSCs) delivery is a non-invasive method that has received interests for treatment of neurodegenerative diseases, such as multiple sclerosis (MS). The impact of intranasal MSCs on intermittent cuprizone model of demyelination was a focus of this study. C57/BL6 mice were fed with 0.3% cuprizone in an intermittent or single ways. Luxol fast blue (LFB), Rotarod test, quantitative real‐time polymerase chain reaction (qRT-PCR), immunohistochemistry and western blot (WB) were used for interpretation of outcomes. MSCs effectively homed to the corpus callosum area, were able to improve motor coordination and to promote myelin recovery in the intermittent cuprizone (INTRCPZ/MSCs). Astrogliosis (GFAP+ cells) and microgliosis (Iba-1+ cells) were hampered, and more mature oligodendrocyte cells (APC+ cells) were identified in mice receiving INTRCPZ/MSCs. Such treatment also considerably reduced markers related to the macrophage type 1 (M1) cells, namely iNOS and CD86, but it recovered the M2 markers MRC-1 and TREM-2. In addition, a remarkable decrease in the expressions of pro-inflammatory IL-1β and TNFα but an increase in the rate of anti-inflammatory TGF-β and IL-10 were identified in mice that underwent INTRCPZ/MSCs therapy. Finally, microvascular changes were evaluated, and a noticeable increase in the expression of the endothelial cell marker CD31 was found in the INTRCPZ/MSCs-treated mice (p < 0.05 for all). The outcomes are representative of the efficacy of intranasal MSCs delivery in intermittent cuprizone model of MS for reshaping macrophage polarity along with modification of glial, inflammatory, and angiogenic markers in favor of therapy. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Related Docs
View other Related Docs
1. Microglia/Macrophage Polarization Regulates Spontaneous Remyelination in Intermittent Cuprizone Model of Demyelination, Biochemistry and Biophysics Reports (2024)
2. Mesenchymal Stem Cell Mediated Effects on Microglial Phenotype in Cuprizone-Induced Demyelination Model, Journal of Cellular Biochemistry (2019)
3. Benefits of Bone Marrow Mesenchymal Stem Cells Compared to Their Conditioned Medium in Valproic Acid-Induced Autism in Rats, Molecular Biology Reports (2024)
4. Current Advances in Stem Cell Therapy in the Treatment of Multiple Sclerosis, Reviews in the Neurosciences (2023)
5. The Effect of Microglial Ablation and Mesenchymal Stem Cell Transplantation on a Cuprizone-Induced Demyelination Model, Journal of Cellular Physiology (2021)
6. Mesenchymal Stromal Cell-Derived Secretome-Based Therapy for Neurodegenerative Diseases: Overview of Clinical Trials, Stem Cell Research and Therapy (2023)
7. Intranasal Delivery of Sdf-1Α-Preconditioned Bone Marrow Mesenchymal Cells Improves Remyelination in the Cuprizone-Induced Mouse Model of Multiple Sclerosis, Cell Biology International (2020)
8. Effect of Mesenchymal Stem Cells on Glial Cells Population in Cuprizone Induced Demyelination Model, Neuropeptides (2019)
Experts (# of related papers)
View all Related Experts
Iraj Ragerdi Kashani (8)
Parichehr Pasbakhsh (7)
Other Related Docs
9. Melatonin Application in Targeting Oxidative-Induced Liver Injuries: A Review, Journal of Cellular Physiology (2018)
10. Adipose Tissue-Derived Mesenchymal Stem Cells Exert in Vitro Immunomodulatory and Beta Cell Protective Functions in Streptozotocin-Induced Diabetic Mice Model, Journal of Diabetes Research (2015)
11. Roles of Methyltrienolone (R1881) in Akts and Ar Expression Patterns of Cultured Granulosa-Lutein Cells, Journal of Cellular Biochemistry (2018)
12. Therapeutic Potential of Stem Cells for Treatment of Neurodegenerative Diseases, Biotechnology Letters (2020)
13. The Comparative Effects of Schwann Cells and Wharton's Jelly Mesenchymal Stem Cells on the Aim2 Inflammasome Activity in an Experimental Model of Spinal Cord Injury, Neuroscience (2023)
14. Metformin Therapy Attenuates Pro-Inflammatory Microglia by Inhibiting Nf-Κb in Cuprizone Demyelinating Mouse Model of Multiple Sclerosis, Neurotoxicity Research (2021)
15. Resveratrol Pretreatment Enhanced Homing of Sdf-1Α-Preconditioned Bone Marrow-Derived Mesenchymal Stem Cells in a Rat Model of Liver Cirrhosis, Journal of Cellular Biochemistry (2018)
16. Combination Effects of Mesenchymal Stem Cells Transplantation and Anodal Transcranial Direct Current Stimulation on a Cuprizone-Induced Mouse Model of Multiple Sclerosis, Journal of Molecular Histology (2022)
17. Targeting Axonal Degeneration and Demyelination Using Combination Administration of 17Β-Estradiol and Schwann Cells in the Rat Model of Spinal Cord Injury, Journal of Cellular Biochemistry (2018)
18. Recent Advances in Mesenchymal Stem Cell Therapy for Multiple Sclerosis: Clinical Applications and Challenges, Frontiers in Cell and Developmental Biology (2025)
19. Mesenchymal Stromal Cells (Mscs) for Neurodegenerative Disease: A Promising Frontier, European Journal of Cell Biology (2020)
20. Manipulated Mesenchymal Stem Cells Applications in Neurodegenerative Diseases, International Journal of Stem Cells (2020)
21. Post-Treatment of Melatonin With Ccl4 Better Reduces Fibrogenic and Oxidative Changes in Liver Than Melatonin Co-Treatment, Journal of Cellular Biochemistry (2018)
22. Toll-Like Receptors in Alzheimer's Disease, Journal of Neuroimmunology (2020)
23. Inflammasome: Its Role in Traumatic Brain and Spinal Cord Injury, Journal of Cellular Physiology (2018)
24. Calorie Restriction Promotes Remyelination in a Cuprizone-Induced Demyelination Mouse Model of Multiple Sclerosis, Metabolic Brain Disease (2020)