Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones

Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones Publisher

Moghadam SO¹ ; Afshar D² ; Nowroozi MR¹ ; Behnamfar A¹ ; Farzin A¹

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1. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Microbiology and Virology, Zanjan University of Medical Sciences, Zanjan, Iran

Source: Infection and Drug Resistance Published:2020

Abstract

Purpose: Given the importance of treatment failure due to multidrug-resistant (MDR) strains, studies on population structure of these organisms are necessary to improve control strategies. Accordingly, the current study aimed to determine the prevalence of carbapenemresistant P. aeruginosa (CRPA) at a teaching referral hospital in Iran and to analyze their molecular clonality by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) for epidemiological purposes. Methods: In this study, modified Hodge test (MHT) and double-disk synergy test (DDST) were used for carbapenemase production and metallo-β-lactamases (MBLs) screening, respectively. All P. aeruginosa isolates were tested for antimicrobial resistance. Moreover, MBL genes (blaIMP, blaVIM, blaSPM, blaNDM) were detected by multiplex PCR assay. Results: Among 68 P. aeruginosa clinical isolates, 38 (55.88%) isolates were CRPA. Antibiotic susceptibility testing revealed that most of these isolates were MDR. PFGE analyses showed 5 common types and 27 single types among CRPA isolates. MLST analysis revealed three major clusters (MLST-sequence types (STs): 235, 357, and 861) among them. The 30 non-CRPA isolates corresponded mainly to MLST-STs 253, 360, and 446. Conclusion: Our results showed that internationally distributed MLST-STs with widely genomic diversity have spread in our hospital, and clonal expansion of MDR strains of P. aeruginosa was described as well. © 2020 Ohadian Moghadam et al.

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Style	Citing Format
MLA	Moghadam SO, et al.. "Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones." Infection and Drug Resistance, vol. 13, no. , 2020, pp. 1583-1592.
APA	Moghadam SO, Afshar D, Nowroozi MR, Behnamfar A, Farzin A (2020). Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones. Infection and Drug Resistance, 13(), 1583-1592.
Chicago	Moghadam SO, Afshar D, Nowroozi MR, Behnamfar A, Farzin A. "Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones." Infection and Drug Resistance 13, no. (2020): 1583-1592.
Harvard	Moghadam SO et al. (2020) 'Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones', Infection and Drug Resistance, 13(), pp. 1583-1592.
Vancouver	Moghadam SO, Afshar D, Nowroozi MR, Behnamfar A, Farzin A. Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones. Infection and Drug Resistance. 2020;13():1583-1592.
BibTex	@article{ author = {Moghadam SO and Afshar D and Nowroozi MR and Behnamfar A and Farzin A}, title = {Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones}, journal = {Infection and Drug Resistance}, volume = {13}, number = {}, pages = {1583-1592}, year = {2020} }
RIS	TY - JOUR AU - Moghadam SO AU - Afshar D AU - Nowroozi MR AU - Behnamfar A AU - Farzin A TI - Molecular Epidemiology of Carbapenemase-Producing Pseudomonas Aeruginosa Isolated From an Iranian University Hospital: Evidence for Spread of High-Risk Clones JO - Infection and Drug Resistance VL - 13 IS - SP - 1583 EP - 1592 PY - 2020 ER -

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