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Blood Culture-Negative Endocarditis Caused by Bartonella Quintana in Iran Publisher Pubmed



Azimzadeh M1 ; Alikhani MY1, 2 ; Sazmand A3 ; Saberi K4 ; Farahani Z5 ; Kamali M6 ; Haddadzadeh M7 ; Safarpoor G8 ; Nourian A3 ; Mohammadi Y9 ; Beikpour F10 ; Salehi M11 ; Greco G12 ; Chomel B13
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan University of Medical Sciences, Hamadan, Iran
  3. 3. Department of Pathobiology, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, 6517658978, Iran
  4. 4. Department of Anesthesia, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Nursery, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Infectious Diseases, Shaheed Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Cardiac Surgery, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, 8915887856, Iran
  8. 8. Department of Cardiovascular Surgery, School of Medicine, Farshchian Cardiovascular Subspecialty Medical Center, Hamadan University of Medical Sciences, Hamadan, Iran
  9. 9. Department of Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran
  10. 10. Department of Pediatrics, School of Medicine, Washington University, St. Louis, 63110, MO, United States
  11. 11. Department of Cardiovascular and Transplantation Surgery, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
  12. 12. Department of Veterinary Medicine, University of Bari “Aldo Moro”, Valenzano, Bari, 70010, Italy
  13. 13. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, United States

Source: Scientific Reports Published:2024


Abstract

Blood culture-negative endocarditis (BCNE) is a challenging disease because of the significant impact of delayed diagnosis on patients. In this study, excised heart valves and blood serum samples were collected from 50 BCNE patients in two central hospitals in Tehran, Iran. Sera were tested by IFA for the presence of IgG and IgM antibodies against Bartonella quintana and B. henselae. Genomic DNA extracted from the heart valves was examined for Bartonella-specific ssrA gene in a probe-based method real-time PCR assay. Any positive sample was Sanger sequenced. IgG titer higher than 1024 was observed in only one patient and all 50 patients tested negative for Bartonella IgM. By real-time PCR, the ssrA gene was detected in the valve of one patient which was further confirmed to be B. quintana. Bartonella-like structures were observed in transmission electron microscopy images of that patient. We present for the first time the involvement of Bartonella in BCNE in Iran. Future research on at-risk populations, as well as domestic and wild mammals as potential reservoirs, is recommended. © The Author(s) 2024.
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1. Molecular Investigation of Coxiella Burnetii and Bartonella in Heart Valve Specimens of Patients With Endocarditis in Iran, Journal of Infection and Public Health (2025)
2. Infective Endocarditis; Report From a Main Referral Teaching Hospital in Iran, Iranian Journal of Pharmaceutical Research (2017)
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