Ternary Poly(3,4-Ethylenedioxythiophene) Polystyrene Sulfonate/ Ti3c2tx/ Silver Nanowire Electrochemical Aptasensor for Bisphenol a Detection Publisher



Eshtiaghi M ; Salekmaghsoudi AS ; Khodarahmi A ; Ramezanzadeh B ; Pishbin F ; Hassani S
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects Published:2025

Abstract

The incorporation of advanced materials into sensing systems helps address existing challenges, enabling ultrasensitive detection of analytes in complex media. This study introduces a ternary PEDOT:PSS/Ti₃C₂Tx/AgNW biosensor for ultrasensitive aptasensing of bisphenol A (BPA). Fabrication of the nanocomposite is validated through XRD, FESEM, XPS, and Raman spectroscopy, confirming successful structural and morphological integration. EIS and CV analyses demonstrate improved charge transfer, with Rct decreasing from 7.2 × 10² Ω·cm² in AgNW (A) to 3.4 × 10² Ω·cm² in Ti₃C₂Tx/AgNW (MA), and 2.8 Ω·cm² in PEDOT:PSS/Ti₃C₂Tx/AgNW (PMA). Increased Rct and reduced CV peaks from 300.5 μA in PMA to 194.3 μA in aptamer/PMA (APMA) and 174.6 μA in BPA/APMA confirm aptamer immobilization and BPA binding. DPV results show a detection range from 1 μM to 1 pM and a LOD of 0.7 pM. Selectivity is confirmed in the presence of structural analogues (HBPA, BPB, estradiol), with strong durability over 30 days, repeatability across six electrodes, and electrochemical stability over 1000 cycles. Finally, favorable recovery values and low relative standard deviations (RSDs) (<1 % and <3 %) for BPA detection in mineral water underscore the aptasensor's accuracy and precision, demonstrating its strong potential for detecting BPA in contaminated real-world samples. © 2025 Elsevier B.V., All rights reserved.