Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Ifnar2 and Tyk2 Transcripts’ Prognostic Role in Covid-19 Patients: A Retrospective Study Publisher Pubmed



Razavi A1, 2 ; Raei M3 ; Hatami Y4 ; Chokami GS4 ; Goudarzi Y4 ; Ghasemian R5 ; Alizadehnavaei R3 ; Yarmohammadi H6 ; Soltanipur M6 ; Tabarestani M1 ; Valadan R7, 8 ; Meshkinfam Haghighi F9 ; Tarsi AK7, 10 ; Razavi B11
Authors
Show Affiliations
Authors Affiliations
  1. 1. Student Research Committee, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. Research Center for Clinical Virology, Tehran University of Medical Science, Tehran, Iran
  3. 3. Gastrointestinal Cancer Research Center, Non-Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Central Human Immunodeficiency Virus Laboratory, Mazandaran University of Medical Sciences, Sari, Iran
  5. 5. Antimicrobial Resistance Research Center, Department of Infectious Diseases, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  6. 6. Medical Students Research Committee, Shahed University, Tehran, Iran
  7. 7. Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  8. 8. Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  9. 9. Department of Psychiatry, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Department of Clinical Biochemistry and Genetics, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  11. 11. Medical Research Center, Islamic Azad University, Tehran, Iran

Source: Frontiers in Cellular and Infection Microbiology Published:2024


Abstract

Background and objectives: This study aimed to investigate the possible prognostic significance of interferon alpha–beta receptor subunit 2 (IFNAR2) and tyrosine kinase 2 (TYK2) expressions. Methods: We conducted a retrospective study including COVID-19 adult patients. All blood samples were collected before any interventions. The expressions of IFNAR2 and TYK2 were assessed using real-time PCR in venous blood samples of 54 cases and 56 controls. The transcript quantities of IFNAR2 and TYK2 genes were assessed using a Delta-Ct method. Results: Our findings show no significant differences in gene expression levels for IFNAR2 and TYK2 between patients who required oxygen (O2) therapy and those who did not (p-value = 0.732 and p-value = 0.629, respectively). Likewise, there were no significant differences in IFNAR2 and TYK2 expressions between patients hospitalized for less than 7 days and those hospitalized for 7 days or more (p-value = 0.455 and p-value = 0.626, respectively). We also observed a weak correlation between IFNAR2 expression and CRP (p-value = 0.045, r = 0.192). There was a negative correlation between the expression levels of IFNAR2 and TYK2 transcripts in COVID-19 patients (p-value = 0.044; partial correlation coefficient = -0.283). Additionally, IFNAR2 and TYK2 were significantly downregulated in the COVID-19 group compared to healthy subjects (p-value = 0.002 and p-value = 0.028, respectively). However, neither IFNAR2 nor TYK2 expression was significantly different between the case subgroups based on COVID-19 severity. The IFNAR2 ΔΔCt (B = -0.184, 95% CI: -0.524–0.157, p-value = 0.275) and the TYK2 ΔΔCt (B = 0.114, 95% CI: -0.268–0.496, p-value = 0.543) were not found to be significant predictors of hospitalization duration. The area under the curve (AUC) for IFNAR2 expression is 0.655 (p-value = 0.005, 95% CI: 0.554–0.757), suggesting its poor discriminative value. Conclusion: We were unable to comment definitively on the prognostic power of IFNAR2 and TYK2 expressions in COVID-19 patients, and larger-scale studies are needed. The principal limitations of this study included the lack of longitudinal analysis and limited sample size. Copyright © 2024 Razavi, Raei, Hatami, Chokami, Goudarzi, Ghasemian, Alizadeh-Navaei, Yarmohammadi, Soltanipur, Tabarestani, Valadan, Meshkinfam Haghighi, Tarsi and Razavi.
Related Docs
1. Pathological Effects of Sars-Cov-2 on Hematological and Immunological Cells: Alterations in Count, Morphology, and Function, Pathology Research and Practice (2022)
2. Immunology, Immunopathogenesis and Immunotherapeutics of Covid-19; an Overview, International Immunopharmacology (2021)
3. Flavonoids As Promising Antiviral Agents Against Sars‐Cov‐2 Infection: A Mechanistic Review, Molecules (2021)
Experts (# of related papers)
View all Related Experts
Azadeh Omidkhoda (1)
Roja Rahimi (1)