In Vitro Antibacterial Activity Study of Doxycycline/Polymethacrylic Acid Coated Silk Suture Publisher



Khanmohammadi A¹ ; Hassani M² ; Vahabi S³ ; Salman BN⁴ ; Ramazani A⁵
Source: Fibers and Polymers Published:2022

Abstract

One of the challenging issues in surgery is a surgical-site infection (SSI), which results in impaired healing and can be life-threatening. Sutures can increase the risk of infection by providing an area for adherent bacteria. In order to reduce the risk of infection caused by sutures, several types of suture coatings have been developed. Hence, doxycycline (DOXY) was applied as an antibiotic for coating silk sutures to evaluate its antimicrobial potential. This study focuses on the coating of a silk suture based on nanohydrogel carreier polymethacrylic acid (PMAA) for the delivery of DOXY. For this purpose, the structure and morphology of the designed nanohydrogel carreier and coated suture were investigated by several descriptive methods such as Fourier Transform Infrared Spectroscopy (FTIR), X-ray difraction (XRD) analysis, and scanning electron microscopy (SEM). The maximum amount of drug loading (DL) and encapsulation efficiency (EE) by nanohydrogel was 6.25 % and 29 %, respectively and DL for coated silk suture was 1.37 % per cm. The release of drug of the coated silk suture occurred in a time-dependent manner, which is favorable for the intended use. In addition, in vitro antibacterial potential of the coated suture was evaluated. Comparing their zones of inhibition showed that the Escherichia coli was more inhibited than the Staphylococcus aureus bacteria. Our results were indicated that DOXY-loaded PMAA coated silk suture can noticeably impede bacterial adhesion. © 2022, The Korean Fiber Society.