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Preconditioning Adipose-Derived Stem Cells With Photobiomodulation Significantly Increased Bone Healing in a Critical Size Femoral Defect in Rats Publisher Pubmed



Khosravipour A1 ; Amini A1 ; Masteri Farahani R1 ; Zare F1 ; Mostafavinia A2 ; Fallahnezhad S3 ; Akbarzade S2 ; Asgari M1 ; Mohammadbeigi A4 ; Rezaei F5 ; Ghoreishi SK6 ; Chien S7 ; Bayat M1, 7
Authors
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Authors Affiliations
  1. 1. Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  3. 3. Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  4. 4. Department of Radiology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. University of Kentucky College of Pharmacy 789 South Limestone Lexington, Kentucky, 40536, United States
  6. 6. Department of Statistics, Qom University, Qom, Iran
  7. 7. Price Institute of Surgical Research, University of Louisville, and Noveratech LLC, Louisville, KY, United States

Source: Biochemical and Biophysical Research Communications Published:2020


Abstract

We assessed the combined impacts of human demineralized bone matrix (hDBM) scaffold, adipose-derived stem cells (hADS), and photobiomodulation (PBM) on bone repair of a critical size femoral defect (CSFD) in 72 rats. The rats were divided into six groups: control (group 1); ADS (group 2 - ADS transplanted into hDBM); PBM (group 3 - PBM-treated CSFDs); ADS + PBM in vivo (group 4 - ADS transplanted into hDBM and the CSFDs were treated with PBM in vivo); ADS + PBM in vitro (group 5 - ADS were treated with PBM in vitro, then seeded into hDBM); and ADS + PBM in vitro+in vivo (group 6 - PBM-treated ADS were seeded into hDBM, and the CSFDs were treated with PBM in vivo. At the anabolic phase (2 weeks after surgery), bone strength parameters of the groups 5, 6, and 4 were statistically greater than the control, ADS, and PBM in vivo groups (all, p = 0.000). Computed tomography (CT) scans during the catabolic phase (6 weeks after surgery) of bone healing revealed that the Hounsfield unit (HU) of CSFD in the groups 2 (p = 0.000) and 5 (p = 0.019) groups were statistically greater than the control group. The groups 5, 4, and 6 had significantly increased bone strength parameters compared with the PBM in vivo, control, and ADS groups (all, p = 0.000). The group 5 was statistically better than the groups 4, and 6 (both, p = 0.000). In vitro preconditioned of hADS with PBM significantly increased bone repair in a rat model of CSFD in vivo. © 2020 Elsevier Inc.
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