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Cytoplasmic Expression of B7-H3 and Membranous Epcam Expression Are Associated With Higher Grade and Survival Outcomes in Patients With Clear Cell Renal Cell Carcinoma Publisher Pubmed



Saeednejad Zanjani L1 ; Madjd Z1, 2 ; Axcrona U3 ; Abolhasani M1, 4 ; Rasti A1, 5 ; Asgari M1, 4 ; Fodstad O6, 7 ; Andersson Y6
Authors
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Authors Affiliations
  1. 1. Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
  2. 2. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Pathology, The Norwegian Radium Hospital, Institute of Clinical Medicine, Oslo University Hospital, Faculty of Medicine, University of Oslo, Oslo, Norway
  4. 4. Hasheminejad Kidney Center, Iran University of Medical Sciences, (IUMS), Tehran, Iran
  5. 5. Department of Basic Sciences/Medical Surgical Nursing, Faculty of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Tumor Biology, Norwegian Radium Hospital, Oslo University Hospital Radiumhospitalet, Oslo, Norway
  7. 7. Faculty of Medicine, Institute of Clinical Medicine, University of Oslo, Oslo, Norway

Source: Annals of Diagnostic Pathology Published:2020


Abstract

B7-H3 and EpCAM are overexpressed in cancer and play a role in tumorigenesis and metastasis. In this study, the membranous, cytoplasmic and nuclear expression levels of B7-H3 and EpCAM biomarkers were mapped in three major subtypes of renal cell carcinoma (RCC). Expression of B7-H3 and EpCAM were evaluated using immunohistochemistry in RCC samples on tissue microarrays (TMAs), including clear cell RCCs (ccRCCs), type I and II papillary RCCs (pRCCs), and chromophobe RCCs (chRCCs). The association between B7-H3 and EpCAM expression and clinicopathological features as well as survival outcomes was determined. There was a statistically significant difference between B7-H3 and EpCAM expression among the different RCC subtypes. In ccRCC, higher cytoplasmic expression of B7-H3 was significantly associated with increase in nucleolar grade, lymph node invasion (LNI), invasion of the Gerota's fascia, and tumor necrosis, while no association was found with the membranous and nuclear expression. Moreover tumors with cytoplasmic expression of B7-H3 tended to have a worse prognosis for disease-specific survival (DSS) than those with membranous expression. In case of EpCAM, increased membranous expression of EpCAM was associated with nucleolar grade and tumor necrosis in ccRCC. Additionally, membranous EpCAM expression added prognostic value in patients with ccRCC who had high nucleolar grade versus low nucleolar grade. Moreover, membranous EpCAM expression was found to be an independent favorable prognostic marker for progression-free survival (PFS) in ccRCC. Our results demonstrated that higher cytoplasmic B7-H3 and membranous EpCAM expression are clinically significant in ccRCC and are associated with more aggressiveness tumor behavior. © 2020 Elsevier Inc.
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