Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients

Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients Publisher Pubmed

Piran M¹ ; Enderami SE¹ ; Piran M¹ ; Sedeh HS¹ ; Seyedjafari E² ; Ardeshirylajimi A³

Show Affiliations

1. Stem Cell Technology Research Center, Tehran, Iran
2. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
3. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Biologicals Published:2017

Abstract

Diabetes Mellitus (DM) is a systematic disease, which happens because of destruction of islets of Langerhans in the pancreas and systematic resistance to insulin. The lack of donor for pancreas transplantation and risk of transplant rejection is the main challenge in the treatment of this disease. Stem cells are proper and sufficient source for creating insulin-producing cells (IPC). In this study adipose tissue was provided from diabetic patients operated for liposuction and then adipose derived stem cells (ADSCs) were isolated, characterized and then treated by lentiviruses containing miR-375, after 7, 14 and 21 days of induction, islet-like clusters (ILC) specific genes including insulin and PDX1 were evaluated by Real Time RT-PCR. Finally, immunocytochemistry was also used for evaluation of these markers in the protein level. The results were shown that insulin and PDX1 genes and proteins expression significantly increased in transduced stem cells compared to the control group. According to the results it can be concluded that islet-like clusters can be achieved from ADSCs by overexpression of miR-375. © 2016 International Alliance for Biological Standardization

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Style	Citing Format
MLA	Piran M, et al.. "Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients." Biologicals, vol. 46, no. , 2017, pp. 23-28.
APA	Piran M, Enderami SE, Piran M, Sedeh HS, Seyedjafari E, Ardeshirylajimi A (2017). Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients. Biologicals, 46(), 23-28.
Chicago	Piran M, Enderami SE, Piran M, Sedeh HS, Seyedjafari E, Ardeshirylajimi A. "Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients." Biologicals 46, no. (2017): 23-28.
Harvard	Piran M et al. (2017) 'Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients', Biologicals, 46(), pp. 23-28.
Vancouver	Piran M, Enderami SE, Piran M, Sedeh HS, Seyedjafari E, Ardeshirylajimi A. Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients. Biologicals. 2017;46():23-28.
BibTex	@article{ author = {Piran M and Enderami SE and Piran M and Sedeh HS and Seyedjafari E and Ardeshirylajimi A}, title = {Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients}, journal = {Biologicals}, volume = {46}, number = {}, pages = {23-28}, year = {2017} }
RIS	TY - JOUR AU - Piran M AU - Enderami SE AU - Piran M AU - Sedeh HS AU - Seyedjafari E AU - Ardeshirylajimi A TI - Insulin Producing Cells Generation by Overexpression of Mir-375 in Adipose-Derived Mesenchymal Stem Cells From Diabetic Patients JO - Biologicals VL - 46 IS - SP - 23 EP - 28 PY - 2017 ER -

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