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The Generation of Islet-Like Insulin-Producing Cells From Wharton's Jelly-Derived Mesenchymal Stem Cells on the Pes/Fish Gelatin Scaffold Publisher



Soleimanifar F1 ; Aghapur N2 ; Rezaeikiasari Z3 ; Mahboudi H1 ; Kaabi M2 ; Mansour RN4 ; Kehtari M5 ; Abazari M6 ; Enderami SE7 ; Hassannia H8
Authors
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Authors Affiliations
  1. 1. Department of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
  2. 2. Stem Cell Research Technology, Tehran, Iran
  3. 3. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Department of Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  5. 5. Department of Animal Biology, School of Biology, Faculty of Science, University of Tehran, Tehran, Iran
  6. 6. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Diabetes Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  8. 8. Amol Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran

Source: Regenerative Therapy Published:2024


Abstract

Diabetes Mellitus (DM) disrupts the body's capability to control blood glucose statuses. Type 1 diabetes mellitus (T1DM) arises from inadequate insulin production and is treated with insulin replacement therapy. Stem cell therapy is a hopeful treatment for T1DM that involves using adult stem cells to generate insulin-producing cells (IPCs). Mesenchymal stem cells (MSCs) are particularly advantageous for generating IPCs. The islet cells require interactions with the extracellular matrix for survival, which is lacking in conventional 2D culture systems. Natural or synthetic polymers create a supportive 3D microenvironment in tissue engineering. We aim to construct superior differentiation conditions employing polyethersulfone (PES)/Fish gelatin scaffolds to differentiate Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) to IPCs. In this study, the PES/fish gelatin scaffold (3D) was manufactured by electrospinning, and then its biocompatibility and non-toxicity were investigated by MTT assay. After that, scaffold-supportive effects on WJ-MSCs differentiation to IPCs were studied at the gene and protein levels. After exposure to the differentiation media, 2D and 3D (PES/Fish gelatin) cultured cells were slowly aggregated and developed spherical-shaped clusters. The viability of cells was found to be comparable in both 2D and 3D cultures. The gene expression analysis showed that efficiency of differentiation was more elevated in 3D culture. Additionally, ELISA results indicated that C-peptide and insulin release were more significant in 3D than in 2D culture. In conclusion, the PES/fish gelatin scaffold is highly promising for pancreatic tissue engineering because it supports the viability, growth, and differentiation of WJ-MSCs into IPCs. © 2024 The Japanese Society for Regenerative Medicine
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