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Mir-181B and Mir-204 Suppress the Vsmc Proliferation and Migration by Downregulation of Hck Publisher Pubmed



Ghasempour G1, 2 ; Mahabadi VP3, 4 ; Shabani M1 ; Mohammadi A5 ; Zamanigarmsiri F6 ; Amirfarhangi A7 ; Karimi M8 ; Najafi M1, 4
Authors
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Authors Affiliations
  1. 1. Clinical Biochemistry Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Student Research Committee, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Neuroscience Research Center, Vice-Chancellor for Research and Technology, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Clinical Biochemistry Department, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
  6. 6. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Hazrat-E-Rasool Hospital, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Microvascular Research Published:2021


Abstract

Background: VSMC proliferation and migration pathways play important roles in plaque formation in the vessel stenosis and re-stenosis processes. The microRNAs affect the expression of many genes that regulate these cellular processes. The aim of this study was to investigate the effects of miR-181b, miR-204, and miR-599 on the gene and protein expression levels of hematopoietic cell kinase (HCK) in VSMCs. Methods: miR-181b, miR-204 were predicted for the suppression of HCK in the chemokine signaling pathway using bioinformatics tools. Then, the VSMCs were transfected by PEI-containing microRNAs. The HCK gene and protein expression levels were evaluated using RT-qPCR and Western blotting techniques, respectively. Moreover, the cellular proliferation and migration were evaluated by MTT and scratch assay methods. Results: The miR-181b and miR-204 decreased significantly the HCK gene and (total and phosphorylated) protein expression levels. Also, the miR-599 did not show any significant effects on the HCK gene and protein levels. The data also showed that miR-181b, miR-204, and miR-599 prevent significantly the proliferation and migration of VSMCs. Conclusion: The downregulation of HCK by miR-181b and miR-204 suppressed the VSMC proliferation and migration. © 2021
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