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Molecular Diagnosis of Human Cutaneous Leishmaniasis and Identification of the Causative Leishmania Species in Iran: A Narrative Review Publisher Pubmed



Eskandari SE1 ; Memariani M2, 3 ; Memariani H4 ; Mohebali M5, 6 ; Khamesipour A1
Authors
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Authors Affiliations
  1. 1. Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  5. 5. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Centers for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian biomedical journal Published:2024


Abstract

Cutaneous leishmaniasis (CL) is a common form of leishmaniasis in underdeveloped countries. Although CL tends to be self-limiting, it can cause significant scars and may progress to more severe manifestations. Additionally, Leishmania species vary in susceptibility to the available treatments. The selection of treatment and clinical outcome of CL depend on the accurate determination of the Leishmania species. This mini-review aims to provide an overview of the molecular diagnosis techniques such as PCR-based assays, nucleic acid sequence-based amplification, and loop-mediated isothermal amplification utilized in the identification of Leishmania species in Iran.