The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii

The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii Publisher Pubmed

Badmasti F^{1, 2} ; Habibi M³ ; Firoozeh F⁴ ; Fereshteh S¹ ; Bolourchi N¹ ; Goodarzi NN⁵

Show Affiliations

1. Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
2. Microbiology Research Center (MRC), Pasteur Institute of Iran, Iran
3. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
4. Department of Microbiology, School of Medicine, Alborz University of Medical Science, Karaj, Iran
5. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Microbial Pathogenesis Published:2021

Abstract

Due to the emergence of multi-drug resistant Acinetobacter baumannii strains, there is an urgent need to develop several new strategies to control this bacterium. In this context, vaccination may be the best approach to reduce the morbidity and mortality associated with MDR isolates in vulnerable groups. Serum resistance factors have a key role in the pathogenesis of A. baumannii and can be considered as potential vaccine candidates. This project aimed to evaluate the immunological reactivity of CipA and PBP-7/8 as two serum resistance factors in a combination form against sepsis infections of A. baumannii. Recombinant proteins were obtained and immunological evaluations were performed against sepsis infection in the C57BL/6 mouse model. The data showed a statistically significant increase in total IgG levels in all three immunization regimens (CipA, PBP-7/8, and CipA + PBP-7/8) compared to the control group. The ratios of IgG2c/IgG1 in the CipA, PBP-7/8, and CipA + PBP-7/8 schedules were 8.7, 46.50, and 33.29, respectively. It appears that the immunization schedules developed a strong polarized Th1 response. The cytokine profiles of the three plans showed that IFN-γ was highly concentrated in the combination plan. However, the highest concentration of IL-17 belonged to the PBP-7/8 plan. In conclusion, the data of total IgG, survival rates and splenic bacterial loads showed that the CipA + PBP-7/8 plan was more effective than each protein individually. © 2021 Elsevier Ltd

2. In Silico Analyses of Extracellular Proteins of Acinetobacter Baumannii As Immunogenic Candidates, Iranian Journal of Pharmaceutical Research (2022)

3. Evaluation of Plga Nanoparticles Containing Outer Membrane Proteins of Acinetobacter Baumannii Bacterium in Stimulating the Immune System in Mice, Research in Pharmaceutical Sciences (2022)

4. Identification of Antigens From Nosocomial Acinetobacter Baumannii Clinical Isolates in Sera From Icu Staff and Infected Patients Using the Antigenome Technique, World Journal of Microbiology and Biotechnology (2017)

5. Comparison of Ompa Gene-Targeted Real-Time Pcr With the Conventional Culture Method for Detection of Acinetobacter Baumanii in Pneumonic Balb/C Mice, Iranian Biomedical Journal (2019)

6. In Silico Design and Evaluation of Acinetobacter Baumannii Outer Membrane Protein a (Ompa) Antigenic Peptides As Vaccine Candidate in Immunized Mice, Iranian Journal of Allergy# Asthma and Immunology (2019)

Experts (# of related papers)

Abbas Bahador (1)

Mohammad Hossein Modarressi (1)

Style	Citing Format
MLA	Badmasti F, et al.. "The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii." Microbial Pathogenesis, vol. 158, no. , 2021, pp. -.
APA	Badmasti F, Habibi M, Firoozeh F, Fereshteh S, Bolourchi N, Goodarzi NN (2021). The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii. Microbial Pathogenesis, 158(), -.
Chicago	Badmasti F, Habibi M, Firoozeh F, Fereshteh S, Bolourchi N, Goodarzi NN. "The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii." Microbial Pathogenesis 158, no. (2021): -.
Harvard	Badmasti F et al. (2021) 'The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii', Microbial Pathogenesis, 158(), pp. -.
Vancouver	Badmasti F, Habibi M, Firoozeh F, Fereshteh S, Bolourchi N, Goodarzi NN. The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii. Microbial Pathogenesis. 2021;158():-.
BibTex	@article{ author = {Badmasti F and Habibi M and Firoozeh F and Fereshteh S and Bolourchi N and Goodarzi NN}, title = {The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii}, journal = {Microbial Pathogenesis}, volume = {158}, number = {}, pages = {-}, year = {2021} }
RIS	TY - JOUR AU - Badmasti F AU - Habibi M AU - Firoozeh F AU - Fereshteh S AU - Bolourchi N AU - Goodarzi NN TI - The Combination of Cipa and Pbp-7/8 Proteins Contribute to the Survival of C57bl/6 Mice From Sepsis of Acinetobacter Baumannii JO - Microbial Pathogenesis VL - 158 IS - SP - EP - PY - 2021 ER -

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