Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Antimicrobial Resistance and Molecular Epidemiology of Virulence Genes Among Multi-Drug Resistant Acinetobacter Baumannii Clinical Isolates in Iran Publisher



Sadr M1 ; Fahimzad SA1 ; Karimi A1 ; Fallah F1 ; Armin S1 ; Almasian Tehrani N2 ; Shamshiri AR3 ; Azimi L1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Pediatric Infections Research Center, Research Institute of children's Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran
  3. 3. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Gene Reports Published:2021


Abstract

Introduction: Multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is considered a serious threat level bacterial pathogen. This study aims to investigate the prevalence rate of main virulence genes within clinically MDR isolates of A. baumannii and extract their antibiotic susceptibility patterns. Methods: In the present study, various clinical specimens were collected from general hospitals of Tehran, Iran in two years. The identification of A. baumannii isolates was performed by conventional biochemical tests. The antibiotic resistance profile of A. baumannii isolates was determined using the disk diffusion method and results were interpreted according to the CLSI guidelines. The existence and frequency of main virulence genes were screened by PCR method in MDR A. baumannii isolates. Results: A total of 198 A. baumannii were isolated from clinical specimens in selected general hospitals in Iran, out of which 90 (45.45%) isolates were recognized as MDR A. baumannii isolates. The highest frequency of MDR A. baumannii isolates was detected in blood (42.22%) and urine (24.44%) specimens, respectively. Moreover, most MDR A. baumannii isolates (43.33%) were isolated from ICU. All of MDR A. baumannii isolates were resistant to piperacillin/tazobactam and cefepime. The prevalence of surveyed virulence genes among MDR A. baumannii isolates was as follows: pld (76.67%), bap (70%), traT (66.67%) lasB (56.67%), omp33-36 (52.22%), and ompA (23.33%). Conclusion: The results of our study revealed that the prevalence of MDR A. baumannii isolates and virulence-associated genes in general hospitals was high. It is suggested that virulence genes were associated with antibiotic resistance in A. baumannii isolates. © 2021 Elsevier Inc.
Related Docs
1. Investigation of Epsa, Ompa, and Bap Genes Among Mdr and Xdr Acinetobacter Baumannii Isolates in Khorramabad, Iran, Recent Advances in Anti-Infective Drug Discovery (2024)
2. The Prevalence of Isaba1 and Isaba4 in Acinetobacter Baumannii Species of Different International Clone Lineages Among Patients With Burning in Tehran, Iran, Jundishapur Journal of Microbiology (2015)
3. Prevalence and Molecular Mechanisms of Colistin Resistance in Acinetobacter Baumannii Clinical Isolates in Tehran, Iran, Acta Microbiologica et Immunologica Hungarica (2021)
4. Wide Distribution of Carbapenem Resistant Acinetobacter Baumannii in Burns Patients in Iran, Frontiers in Microbiology (2015)
5. Plasmid Borne Carbapenem-Hydrolyzing Class D Β-Lactamases (Chdls) and Adeabc Efflux Pump Conferring Carbapenem-Tigecycline Resistance Among Acinetobacter Baumannii Isolates Harboring Tnabars, Microbial Pathogenesis (2017)
6. Antimicrobial and Anti-Biofilm Potencies of Dermcidin-Derived Peptide Dcd-1L Against Acinetobacter Baumannii: An in Vivo Wound Healing Model, BMC Microbiology (2022)
7. Genotypic and Antimicrobial Susceptibility of Carbapenem-Resistant Acinetobacter Baumannii: Analysis of Is Aba Elements and Blaoxa-23-Like Genes Including a New Variant, Frontiers in Microbiology (2015)
8. Clonal Relatedness and Resistance Characteristics of Oxa-24 and -58 Producing Carbapenem-Resistant Acinetobacter Baumannii Isolates in Tehran, Iran, Journal of Applied Microbiology (2019)
Experts (# of related papers)
View all Related Experts
Abbas Bahador (5)
Farhad Bonakdar Hashmi (2)