An in Vitro Method to Evaluate Hemolysis of Human Red Blood Cells (Rbcs) Treated by Airborne Particulate Matter (Pm 10 ) Publisher



Mesdaghinia A^{1, 2} ; Pourpak Z³ ; Naddafi K^{1, 2} ; Nodehi RN^{1, 2} ; Alizadeh Z³ ; Rezaei S⁴ ; Mohammadi A⁵ ; Faraji M^{6, 7} Show Affiliations
Source: MethodsX Published:2019

Abstract

Air pollutants are capable to enter bloodstream through the nose, mouth, skin and the digestive tract. Hemolysis is the premature destruction of red blood cells (RBCs) membranes. This can affect metabolism of RBCs and reduce cell life. Each of these adverse effects could lead to anemia, jaundice and other pathological conditions. Hemolysis can induce by the mineral components adsorbed on the particles. The aim of this study was to evaluate hemolysis of RBCs treated by airborne PM 10 (PM with aerodynamic diameter ≤ 10 μm) in vitro. Study had two main stages including sampling and preparation of PM 10 suspension, and hemolysis test. Particle samples were collected by means of a high-volume sampler on fiberglass filters. The PM 10 was extracted through dry ultrasonic method. Blood sample was incubated by PM 10 at concentrations 50–300 μg/mL for 3 h. Hemolysis percent was assessed through measurement of Hemoglobin concentration in test samples and total blood hemoglobin (TBH) sample by the cyanmethemoglobin method. Analysis of variance (ANOVA) and Tukey post-hoc test were applied to compare mean values of hemolysis percent between different PM concentrations. Method used in current study is suggested for investigation of toxic effects of airborne particle matter (PM 1 , PM 2.5 and PM 10 ) on human RBCs. © 2019 The Author(s)