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Clonal Relation and Antimicrobial Resistance Pattern of Extended-Spectrum Β-Lactamase- and Ampc Β-Lactamase-Producing Enterobacter Spp. Isolated From Different Clinical Samples in Tehran, Iran Publisher Pubmed



Ghanavati R1 ; Emaneini M1 ; Kalantarneyestanaki D2 ; Maraji AS1 ; Dalvand M1 ; Beigverdi R1 ; Jabalameli F1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Source: Revista da Sociedade Brasileira de Medicina Tropical Published:2018


Abstract

Introduction: Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. Methods: A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. Results: The most common ESBLs and AmpC β-lactamases were blaTEM (63.3%) and blaEBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. Conclusions: RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates. © 2018, Sociedade Brasileira de Medicina Tropical. All rights reserved.