Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods

Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods Publisher Pubmed

Motamedi M^{1, 2} ; Mirhendi H³ ; Zomorodian K¹ ; Khodadadi H¹ ; Kharazi M⁴ ; Ghasemi Z² ; Shidfar MR² ; Makimura K⁵

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1. Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2. Department of Medical Parasitology and Mycology, School of Public Health, National Institute of Health Research, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Medical Parasitology and Mycology, International Campus, Tehran University of Medical Sciences, Tehran, Iran
5. Laboratory of Space and Environmental Medicine, Graduate School of Medicine, Teikyo University, Tokyo, Japan

Source: Mycoses Published:2017

Abstract

Following our previous report on evaluation of the beta tubulin real-time PCR for detection of dermatophytosis, this study aimed to compare the real-time PCR assay with conventional methods for the clinical assessment of its diagnostic performance. Samples from a total of 853 patients with suspected dermatophyte lesions were subjected to direct examination (all samples), culture (499 samples) and real-time PCR (all samples). Fungal DNA was extracted directly from clinical samples using a conical steel bullet, followed by purification with a commercial kit and subjected to the Taq-Man probe-based real-time PCR. The study showed that among the 499 specimens for which all three methods were used, 156 (31.2%), 128 (25.6%) and 205 (41.0%) were found to be positive by direct microscopy, culture and real-time PCR respectively. Real-time PCR significantly increased the detection rate of dermatophytes compared with microscopy (288 vs 229) with 87% concordance between the two methods. The sensitivity, specificity, positive predictive value, and negative predictive value of the real-time PCR was 87.5%, 85%, 66.5% and 95.2% respectively. Although real-time PCR performed better on skin than on nail samples, it should not yet fully replace conventional diagnosis. © 2017 Blackwell Verlag GmbH

2. A Comparative Study on Morphological Versus Molecular Identification of Dermatophyte Isolates, Journal de Mycologie Medicale (2015)

3. Characterizing the Clinical Isolates of Dermatophytes in Hamadan City, Central West of Iran, Using Pcr-Rlfp Method, Journal de Mycologie Medicale (2018)

4. Translation Elongation Factor 1-Α Gene As a Potential Taxonomic and Identification Marker in Dermatophytes, Medical Mycology (2015)

Style	Citing Format
MLA	Motamedi M, et al.. "Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods." Mycoses, vol. 60, no. 10, 2017, pp. 692-696.
APA	Motamedi M, Mirhendi H, Zomorodian K, Khodadadi H, Kharazi M, Ghasemi Z, Shidfar MR, Makimura K (2017). Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods. Mycoses, 60(10), 692-696.
Chicago	Motamedi M, Mirhendi H, Zomorodian K, Khodadadi H, Kharazi M, Ghasemi Z, Shidfar MR, Makimura K. "Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods." Mycoses 60, no. 10 (2017): 692-696.
Harvard	Motamedi M et al. (2017) 'Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods', Mycoses, 60(10), pp. 692-696.
Vancouver	Motamedi M, Mirhendi H, Zomorodian K, Khodadadi H, Kharazi M, Ghasemi Z, et al.. Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods. Mycoses. 2017;60(10):692-696.
BibTex	@article{ author = {Motamedi M and Mirhendi H and Zomorodian K and Khodadadi H and Kharazi M and Ghasemi Z and Shidfar MR and Makimura K}, title = {Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods}, journal = {Mycoses}, volume = {60}, number = {10}, pages = {692-696}, year = {2017} }
RIS	TY - JOUR AU - Motamedi M AU - Mirhendi H AU - Zomorodian K AU - Khodadadi H AU - Kharazi M AU - Ghasemi Z AU - Shidfar MR AU - Makimura K TI - Clinical Evaluation of Β-Tubulin Real-Time Pcr for Rapid Diagnosis of Dermatophytosis, a Comparison With Mycological Methods JO - Mycoses VL - 60 IS - 10 SP - 692 EP - 696 PY - 2017 ER -

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