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A Comparative Study of Long Interspersed Element-1 Protein Immunoreactivity in Cutaneous Malignancies Publisher Pubmed



Zolfaghari MA1 ; Karimi A1, 2 ; Kalantari E3 ; Korourian A3 ; Ghanadan A4 ; Kamyab K4 ; Esmaili N2, 5 ; Emami Razavi AN6 ; Madjd Z3
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Department of Dermatology, Razi Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Dermatopathology, Razi Dermatology Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Autoimmune Bullous Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Iran National Tumor Bank, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: BMC Cancer Published:2020


Abstract

Background: Skin cancer is the most common cancer worldwide and commonly classified into malignant melanoma (MM) and Nonmelanoma skin cancers (NMSCs), which mainly include basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). The extent to which Long Interspersed Element-1 (LINE-1, L1) ORF1p is expressed in cutaneous malignancies remains to be evaluated. This study aimed to assess LINE-1 ORF1p immunoreactivity in various skin cancer subtypes. Method: The expression level of LINE-1 ORF1p was evaluated in 95 skin cancer specimens comprising 36 (37.9%) BCC, 28 (29.5%) SCC, and 31 (32.6%) melanoma using the tissue microarray (TMA) technique. Then the association between expression of LINE-1 encoded protein and clinicopathological parameters was analyzed. Results: We showed that LINE-1 ORF1p expression level was substantially higher in BCC and SCC patients compared with melanoma samples (p < 0.001). BCC cases had a higher LINE-1 histochemical score (H-score) compared with SCC cases (p = 0.004). In SCC samples, a lower level of LINE-1 ORF1p expression was associated with age younger than the mean (p = 0.041). At the same time, no significant correlation was found between LINE-1 ORF1p expression and other clinicopathological parameters (all p > 0.05). Conclusions: According to our observation, LINE-1 ORF1p immunoreactivity in various skin tumor subtypes extends previous studies of LINE-1 expression in different cancers. LINE-1ORF1p overexpression in NMSCs compared with MM can be considered with caution as a tumor-specific antigen for NMSCs. © 2020 The Author(s).
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