Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase

Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase Publisher

Abedi D¹ ; Fazeli MR² ; Jafarian A¹

Show Affiliations

1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Pharmaceutical Research Published:2004

Abstract

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compared with PGA-450, this immobilized enzyme showed a high synthesis activity. The optimum conditions for synthetic activity was at pH 6, 25°C and 2:6 (6-APA:PGME) substrate ratio. © 2004 by School of Pharmacy Shaheed Beheshti University of Medical Sciences and Health Services.

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Style	Citing Format
MLA	Abedi D, Fazeli MR, Jafarian A. "Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase." Iranian Journal of Pharmaceutical Research, vol. 3, no. 3, 2004, pp. 159-164.
APA	Abedi D, Fazeli MR, Jafarian A (2004). Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase. Iranian Journal of Pharmaceutical Research, 3(3), 159-164.
Chicago	Abedi D, Fazeli MR, Jafarian A. "Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase." Iranian Journal of Pharmaceutical Research 3, no. 3 (2004): 159-164.
Harvard	Abedi D, Fazeli MR, Jafarian A (2004) 'Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase', Iranian Journal of Pharmaceutical Research, 3(3), pp. 159-164.
Vancouver	Abedi D, Fazeli MR, Jafarian A. Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase. Iranian Journal of Pharmaceutical Research. 2004;3(3):159-164.
BibTex	@article{ author = {Abedi D and Fazeli MR and Jafarian A}, title = {Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase}, journal = {Iranian Journal of Pharmaceutical Research}, volume = {3}, number = {3}, pages = {159-164}, year = {2004} }
RIS	TY - JOUR AU - Abedi D AU - Fazeli MR AU - Jafarian A TI - Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase JO - Iranian Journal of Pharmaceutical Research VL - 3 IS - 3 SP - 159 EP - 164 PY - 2004 ER -

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