Optimisation of Candida Rugosa Lipase Esterase Activity

Optimisation of Candida Rugosa Lipase Esterase Activity Publisher

Korbekandi H¹ ; Abedi D² ; Pourhossein M¹ ; Motovalibashi M³ ; Hejazi M² ; Narimousaei M² ; Kabiri M²

Show Affiliations

1. Department of Genetics and Molecular Biology, Isfahan, Iran
2. Department of Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Biology, School of Sciences, Isfahan University, Isfahan, Iran

Source: Biotechnology Published:2008

Abstract

We aimed to optimize esterase production of Candida rugosa lipase (CRL). Active culture of C. rugosa (DSM 2031) was revived and the culture medium containing the most frequently used ingredients was optimized using a fraction of factorial design method, Taguchi. Temperature and pH of the culture was also optimized using one factor at a time method. The optimum combination of the major medium ingredients, in order of their magnitude, was (g L-1): Corn Steep Liquor (CSL) powder, (40), triolein (glyceril trioleate) (10), glucose (0) and oleic acid (2). The optimum temperature and pH were 30°C and 7, appropriately. Using this combination and conditions, esterase activity of the enzyme preparation was increased up to 9 U mL-1, which was equivalent to 20611 U mL-1 of Sigma® lipase lipolytic activity. © 2008 Asian Network for Scientific Information.

Related Docs

1. Efficient Media for High Lipase Production: One Variable at a Time Approach, Avicenna Journal of Medical Biotechnology (2017)

Experts (# of related papers)

Hamid Mirmohammad Sadeghi (1)

Mohammad Rabbani (1)

Style	Citing Format
MLA	Korbekandi H, et al.. "Optimisation of Candida Rugosa Lipase Esterase Activity." Biotechnology, vol. 7, no. 1, 2008, pp. 112-117.
APA	Korbekandi H, Abedi D, Pourhossein M, Motovalibashi M, Hejazi M, Narimousaei M, Kabiri M (2008). Optimisation of Candida Rugosa Lipase Esterase Activity. Biotechnology, 7(1), 112-117.
Chicago	Korbekandi H, Abedi D, Pourhossein M, Motovalibashi M, Hejazi M, Narimousaei M, Kabiri M. "Optimisation of Candida Rugosa Lipase Esterase Activity." Biotechnology 7, no. 1 (2008): 112-117.
Harvard	Korbekandi H et al. (2008) 'Optimisation of Candida Rugosa Lipase Esterase Activity', Biotechnology, 7(1), pp. 112-117.
Vancouver	Korbekandi H, Abedi D, Pourhossein M, Motovalibashi M, Hejazi M, Narimousaei M, et al.. Optimisation of Candida Rugosa Lipase Esterase Activity. Biotechnology. 2008;7(1):112-117.
BibTex	@article{ author = {Korbekandi H and Abedi D and Pourhossein M and Motovalibashi M and Hejazi M and Narimousaei M and Kabiri M}, title = {Optimisation of Candida Rugosa Lipase Esterase Activity}, journal = {Biotechnology}, volume = {7}, number = {1}, pages = {112-117}, year = {2008} }
RIS	TY - JOUR AU - Korbekandi H AU - Abedi D AU - Pourhossein M AU - Motovalibashi M AU - Hejazi M AU - Narimousaei M AU - Kabiri M TI - Optimisation of Candida Rugosa Lipase Esterase Activity JO - Biotechnology VL - 7 IS - 1 SP - 112 EP - 117 PY - 2008 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract