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Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen Publisher Pubmed



Shahbazigahrouei D1 ; Abdolahi M2 ; Zarkeshesfahani SH3 ; Laurent S4 ; Sermeus C4 ; Gruettner C5
Authors
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Authors Affiliations
  1. 1. Department of Medical Physics and Medical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Hezar Jerib Street, Iran
  2. 2. Department of Medical Physics, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
  3. 3. Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan, Hezar Jerib Street, Iran
  4. 4. Department of General, Organic, and Biomedical Chemistry, Molecular Imaging Laboratory, University of Mons, 7000 Mons, 19 Avenue Maistriau, Belgium
  5. 5. Micromod Partikletechnologie GmbH, Rostock, Germany

Source: BioMed Research International Published:2013


Abstract

Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis. © 2013 Daryoush Shahbazi-Gahrouei et al.
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