Cell Cycle Analysis of the Cd133+ and Cd133- Cells Isolated From Human Colorectal Cancer Publisher Pubmed



Gharagozloo M¹ ; Mirzaei HR¹ ; Bagherpour B¹ ; Rezaei A¹ ; Kalantari H² ; Sanei MH³ ; Hosseini M⁴ ; Mohajeri G⁵ ; Tabatabai A⁵ ; Hashemi M⁵
Source: Journal of Cancer Research and Therapeutics Published:2012

Abstract

Aim: The CD133 antigen has been identified as a putative stem cell marker in colorectal cancer tissues. The aim of this study was to investigate the cell cycle state of CD133+ and CD133- cells, isolated from primary human colorectal tumors. Materials and Methods: After mechanical and enzymatic dissociation of the tumor samples, CD133+ and CD133 - subsets were identified and separated by magnetic cell sorting. Flow cytometric analysis was performed to compare the cell cycle of both CD133+ and CD133- cells isolated from primary and liver metastatic cancer cells. Results: The results indicated that CD133+ cells isolated from both primary and liver metastatic colorectal cancers were found in higher percentage in the G0/G1 phases. However, the CD133- cells isolated from primary colorectal cancers were predominantly found in the S and G2/M phases. Surprisingly, the CD133- cells isolated from liver metastatic colorectal cancers were mostly found in the G0/G1 phase. Conclusion: The present study provides evidence that CD133+ cells are in a quiescent state in colorectal cancer, representing a mechanism that would at least partially explain chemotherapy resistance and tumor recurrence in post-Therapy patients.