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The Effect of Lipopolysaccharide on the Expression Level of Immunomodulatory and Immunostimulatory Factors of Human Amniotic Epithelial Cells Publisher Pubmed



Taheri RA1 ; Motedayyen H2 ; Ghotloo S3 ; Masjedi M2 ; Mosaffa N4 ; Mirshafiey A3 ; Saffari M3, 5
Authors
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Authors Affiliations
  1. 1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Pathobiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Microbiology and Laboratory Medicine, Kashan University of Medical Sciences, 5th kilometer of Ravand Road, Kashan, Iran

Source: BMC Research Notes Published:2018


Abstract

Objective: Human amniotic epithelial cells (hAECs) are a novel source of stem cells and have immunomodulatory effects on both the innate and adoptive immune system. hAECs can differentiate into multiple cell lineages that make them a suitable cell source for regenerative medicine. These cells express multiple toll-like receptors (TLRs) and respond to various TLR ligands. This study aimed to evaluate the effect of lipopolysaccharide (LPS), a TLR4 ligand, on the level of immunomodulatory and immunostimulatory factors of hAECs. Results: Our results indicated that LPS had the ability to up-regulate the expression of prostaglandin E2 synthase and transforming growth factor-beta1 in hAECs. However, there was no change in the level of interleukin-1beta, interleukin-6 and interleukin-10 in hAECs when were stimulated with LPS. In addition, we observed tumor necrosis factor-alpha was only expressed at very low level in some of hAECs samples which its expression was independent of the effects of LPS. © 2018 The Author(s).
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