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Cytotoxic Effect of Pluronic F127-Poly (Methyl Vinyl Ether-Alt-Maleic Acid) Micelles Loaded With Doxorubicin on Mcf-7 Cells



Varshosaz J1 ; Hassanzadeh F2 ; Sadeghi Aliabadi H3 ; Larian Z4
Authors
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Authors Affiliations
  1. 1. Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Pharmaceutical Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Biotechnology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Center and Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2013

Abstract

Background: Doxorubicin is used for treatment of different types of cancer including breast cancer. However, there are various side effects such as cardiotoxicity. Using a suitable drug delivery system such as polymeric micelles containing doxorubicin may reduce these effects. Methods: Micelles were prepared by direct dissolution method. After optimization according to their particle size, zeta potential, polydispersity index, loading efficiency and release of doxorubicin, micelles were used for further tests of cytotoxicity on MCF-7 cell line by 3-(4,5-Dimethylthiazol-2yl)-2,5 diphenyltetrazolium bromide (MTT) assay. Findings: The optimum targeted micelles with particle size of 419 ± 38 nm, zeta potential of -13.3 ± 1.2 mv, polydispersity index of 0.45 ± 0.019, loading efficiency of 93.5 ± 2.7 percent and drug release efficiency of 29.03 ± 3.7 percent in different concentrations of 0.21, 0.26, 0.34, 47.0 and 0.78 μM showed higher cytotoxicity than blank micelles and free drug. Conclusion: Pluronic F127-poly (methyl vinyl ether-alt-maleic acid) micelles showed more cytotoxicity than free drug against MCF-7 cell line.
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