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Semaphorin-3A As an Immune Modulator Is Suppressed by Microrna-145-5P Publisher

Summary: Research suggests miR-145-5p lowers SEMA3A, potentially aiding treatment of autoimmune diseases like MS. #AutoimmuneDisease #GeneRegulation

Rezaeepoor M1 ; Ganjalikhanihakemi M1 ; Shapoori S1 ; Eskandari N1 ; Sharifi M2 ; Etemadifar M3 ; Mansuorian M4
Authors

Source: Cell Journal Published:2018


Abstract

Objective: Semaphorin-3A (SEMA3A) and its receptors are found on some immune cells and act as suppressors of immune cells over-activation. Considering the role of SEMA3A and its down-regulation in some autoimmune diseases, as well as our bioinformatics predictions, we assumed that miR-145-5p might affect SEMA3A expression. So, we aimed to determine the effect of miR-145-5p on SEMA3A gene expression level. Materials and Methods: In this experimental study, we evaluated the effect of miR-145-5p transfection on SEMA3A expression in peripheral blood mononuclear cells (PBMCs) using ELISA and quantitative real-time polymerase chain reaction (PCR) methods. Results: Our results showed that miR-145-5p is able to decrease SEMA3A expression at both protein and mRNA levels. These data confirmed our previous bioinformatic prediction about the inhibitory effect of miR-145-5p on SEMA3A expression. Conclusion: These results enlightened us about an unknown aspect of SEMA3A role in some autoimmune disorders like multiple sclerosis (MS) and rheumatoid arthritis (RA) and also proposed SEMA3A as a potential therapeutic approach.
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