Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Reteplase: Structure, Function, and Production Publisher



Mohammadi E1 ; Seyedhosseinighaheh H2 ; Mahnam K3 ; Jahaniannajafabadi A1 ; Mohammad Sadeghi HM1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran
  3. 3. Department of Biology, Faculty of Science, Shahrekord University, Shahr-e Kord, Iran

Source: Advanced Biomedical Research Published:2019


Abstract

Thrombolytic drugs activate plasminogen which creates a cleaved form called plasmin, a proteolytic enzyme that breaks the crosslinks between fibrin molecules. The crosslinks create blood clots, so reteplase dissolves blood clots. Tissue plasminogen activator (tPA) is a well-known thrombolytic drug and is fibrin specific. Reteplase is a modified nonglycosylated recombinant form of tPA used to dissolve intracoronary emboli, lysis of acute pulmonary emboli, and handling of myocardial infarction. This protein contains kringle-2 and serine protease domains. The lack of glycosylation means that a prokaryotic system can be used to express reteplase. Therefore, the production of reteplase is more affordable than that of tPA. Different methods have been proposed to improve the production of reteplase. This article reviews the structure and function of reteplase as well as the methods used to produce it. © 2019 Advanced Biomedical Research.
Related Docs
View other Related Docs
1. Molecular Cloning and Expression of Reteplase in Escherichia Coli Using Tac Promoter, Journal of Isfahan Medical School (2013)
2. Expression of the Recombinant Plasminogen Activator (Reteplase) by a Non-Lytic Insect Cell Expression System, Research in Pharmaceutical Sciences (2013)
3. Bacterial Staphylokinase As a Promising Third-Generation Drug in the Treatment for Vascular Occlusion, Molecular Biology Reports (2020)
4. Design and Production of New Chimeric Reteplase With Enhanced Fibrin Affinity: A Theoretical and Experimental Study, Journal of Biomolecular Structure and Dynamics (2021)
5. Investigation of Supercharging As a Strategy to Enhance the Solubility and Plasminogen Cleavage Activity of Reteplase, Iranian Journal of Biotechnology (2020)
6. Optimization of the Expression of Reteplase in Escherichia Coli Top10 Using Arabinose Promoter, Jundishapur Journal of Natural Pharmaceutical Products (2015)
7. Improving the Solubility, Activity, and Stability of Reteplase Using in Silico Design of New Variants, Research in Pharmaceutical Sciences (2019)
8. Optimization of Fermentation Conditions for Reteplase Expression by Escherichia Coli Using Response Surface Methodology, Avicenna Journal of Medical Biotechnology (2019)
Experts (# of related papers)
View all Related Experts
Hamid Mirmohammad Sadeghi (13)
Mohammad Rabbani (9)
Other Related Docs
9. Rational Design of a New Variant of Reteplase With Optimized Physicochemical Profile and Large-Scale Production in Escherichia Coli, World Journal of Microbiology and Biotechnology (2022)
10. Optimization of the Expression of Reteplase in Escherichia Coli, Research in Pharmaceutical Sciences (2011)
11. Cloning and Expression of a Human Tissue Plasminogen Activator Variant: K2s in Escherichia Coli, Pakistan Journal of Biological Sciences (2007)
12. Cloning and Expression of Functional Reteplase in Escherichia Coli Top10, Avicenna Journal of Medical Biotechnology (2013)
13. Comparison of the Cytoplasmic and Periplasmic Production of Reteplase in Escherichia Coli, Preparative Biochemistry and Biotechnology (2013)
14. Improvement of Soluble Production of Reteplase in Escherichia Coli by Optimization of Chemical Chaperones in Lysis Buffer, Advanced Biomedical Research (2019)
15. Effect of Tat-Signaling Fusion System Along With Co-Expression of Groel/Es Chaperones on Secretory Expression of Somatropin, Biotechnologia (2020)
16. Cloning, Optimization of Periplasmic Expression and Purification of Recombinant Granulocyte Macrophage-Stimulating Factor in Escherichia Coli Bl21 (De3), Advanced Biomedical Research (2019)
17. Twin Arginine Translocation System in Secretory Expression of Recombinant Human Growth Hormone, Research in Pharmaceutical Sciences (2016)
18. Evaluation of Soluble Expression of Recombinant Granulocyte Macrophage Stimulating Factor (Rgm-Csf) by Three Different E. Coli Strains, Research in Pharmaceutical Sciences (2020)
19. Development of a Novel and Efficient Hcdc Process for Heterologous Production of Ambystoma Mexicanum Epidermal Lipoxygenase (Ambloxe) in E. Coli, Iranian Journal of Chemistry and Chemical Engineering (2023)
20. The Assessment of No-Reflow Phenomenon Incidence in Early Versus Delayed Percutaneous Coronary Intervention Following a Primary Fibrinolysis, ARYA Atherosclerosis (2024)
21. High-Yield Production of Granulocyte-Macrophage Colony-Stimulating Factor in E. Coli Bl21 (De3) by an Auto-Induction Strategy, Iranian Journal of Pharmaceutical Research (2019)
22. Exposition of Hepatitis B Surface Antigen (Hbsag) on the Surface of Hek293t Cell and Evaluation of Its Expression, Research in Pharmaceutical Sciences (2016)
23. The Effect of Mutation on Neurotoxicity Reduction of New Chimeric Reteplase, a Computational Study, Research in Pharmaceutical Sciences (2023)
24. Improvement of Solubility and Yield of Recombinant Protein Expression in E. Coli Using a Two-Step System, Research in Pharmaceutical Sciences (2019)
25. Designing a Multifunctional Staphylokinase Variant (Sak-2Rgd-Tti) With Appropriate Thrombolytic Activity in Vitro, Biotechnology Letters (2020)
26. Maximizing the Recovery of the Native P28 Bacterial Peptide With Improved Activity and Maintained Solubility and Stability in Escherichia Coli Bl21 (De3), Journal of Microbiological Methods (2022)
27. Finding Appropriate Signal Peptides for Secretory Production of Recombinant Glucarpidase: An in Silico Method, Recent Patents on Biotechnology (2021)