Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Antigenicity and Immunogenicity of Fused B-Subunit of Heat Labile Toxin of Escherichia Coli and Colonization Factor Antigen I Polyepitopes Publisher Pubmed



Savar NS1 ; Dashti A1 ; Darzi Eslam E1 ; Jahaniannajafabadi A2 ; Jafari A1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
  2. 2. Department of Pharmaceutical Biotechnology, and Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences and Health Services, Isfahan, Iran

Source: Journal of Microbiological Methods Published:2014


Abstract

Linear B-cell epitopes (93AKEFEAAAL101 and 66PQLTDVLN73) of CfaB were genetically fused to ltb-(gly)5-cfaB(1-25). Sera of rabbits immunized with fusion proteins reacted strongly with solid-phase bound ETEC bacteria bearing CFA/I fimbriae. Sera failed to agglutinate or inhibit hemagglutination promoted by CFA/I-positive strain which may be due to solvent inaccessibility of epitope residues on intact fimbriae. © 2014 Elsevier B.V.